细胞穿透肽:一种新型非病毒载体  

Cell Penetrating Peptides:Research Progress of a Novel Non-viral Vectors

作　　者：郭争荣[1,2] 彭焕彦 亢继文[1] 姜慧卿[2] 孙殿兴[1] 

机构地区：[1]中国人民解放军石家庄白求恩国际和平医院全军肝病中心,石家庄050082 [2]河北医科大学第二医院,石家庄050017

出　　处：《中国生物工程杂志》2016年第6期100-106,共7页China Biotechnology

基　　金：国家自然科学基金资助项目(81201297);中国人民解放军总后卫生部研究计划(13QNP012)资助项目

摘　　要：细胞膜的选择通透性对维持细胞内环境的稳定起着非常重要的作用,但细胞膜的这种特性限制了一些生物大分子和药物进入细胞内,不利于对一些细胞内疾病的诊断和药物靶向治疗的应用。如何将一些具有诊断和治疗潜力的生物大分子、药物通过细胞膜进入细胞内一直是医学界研究的热点和难点。细胞穿透肽是一类能够携带多肽、蛋白质、核酸、纳米颗粒、病毒颗粒及药物等穿过细胞膜进入细胞,导致完整载物内化的短肽,为生物大分子和药物进入细胞内部提供了有力的运载工具,其作为载体具有的高转导效率和低毒性特点,已经得到了广泛关注和大量研究。目前,细胞穿透肽作为生物分子和药物细胞内化的运载体已经在荧光成像,肿瘤治疗,抗炎治疗及药物靶向治疗中发挥了潜在的诊断和治疗作用,显示出其诱人的应用前景。The cellular membrane as a selectively permeable barrier plays an important role to maintain a relatively stable intracellular environment.But this limits the entry of biological macromolecules and drug into cells and have a obstacle in diagnosis and treatment of some of intracellular disease and drug-target applications.How to carry biological macromolecules and drugs through the cell membrane into the interior of the cell has also been a hot and difficult medical research.Cell-penetrating peptides（ CPPS） are a class of diverse peptides and can carry polypeptides,proteins,nucleic acids,nanoparticles,virus particles and drugs through the cell membrane into cells,resulting in a complete cargo internalization.CPPS as a carrier with a high transduction efficiency and low toxicity characteristics have gotten widespread concern and lots of researches.These peptides as delivery vectors have a potential diagnostic and therapeutic applications in fluorescence imaging,cancer therapy,anti-inflammatory therapy and targeted therapy drugs.

关 键 词：细胞穿透肽 转导机制 跨膜传递 

分 类 号：Q819[生物学—生物工程]