抗生素含量测定方法的分析概述  

Analysis of Antibiotics Quantification Methods

作　　者：沈伟涛[1] 王明钰[1] 王允坤[2] 王新华[2] 徐海[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100 [2]山东大学环境科学与工程学院,济南250100

出　　处：《中国生物工程杂志》2016年第6期119-126,共8页China Biotechnology

基　　金：国家自然科学基金面上项目(31170112);国家科技重大专项(2013ZX10004217-003);微生物技术国家重点实验室开放课题(M2015-10)资助项目

摘　　要：抗生素作为一种抗菌物质,在现代医疗中起着非常重要的作用。2015版中华人民共和国药典共收录抗生素药品363种,所介绍的抗生素含量测定方法有4种。根据抗生素测定方法的统计,在这363种抗生素中,有275种采用高效液相色谱法;有75种采用抗生素微生物检定法;有8种采用紫外-可见分光光度法;只有5种采用了滴定法。以最新的2015版中华人民共和国药典为基础,对抗生素的测定方法进行了分析概述,期望能在环境抗生素残留量的测定方面提供可借鉴的内容。Being antibacterial chemicals,antibiotics play a very important role in modern medication.The Chinese pharmacopoeia was revised in 2015 which included 363 antibiotics,and introduced four methods to measure the content of antibiotics.A summary of antibiotics measurement methods showed that among the 363 antibiotics,275 antibiotics are measured using high performance liquid chromatography,75 antibiotics are measured using microbial assays,8 antibiotics are measured using ultraviolet-visible spectrophotometry,and only5 antibiotics are measured by titration.A summary of methods for the quantification of the content of antibiotics based on the latest Chinese pharmacopoeia revised in 2015 was provided and analyzed,hoping to provide a reference in the determination of the content of residual environmental antibiotic.

关 键 词：抗生素 中华人民共和国药典 定量技术 高效液相色谱 

分 类 号：R331[医药卫生—人体生理学]