中医药干预对HIV感染者T细胞表面PD-1和Tim-3表达水平的影响  

Intervention of T cell surface PD-1 and Tim-3 expression changes in HIV infected patients by TCM treated

作　　者：杨玉琪[1] 张媛[2] 宋娜丽[1] 王红梅 李秀珍 贺铮铮[1] 孙俊[1] 和丽生[1] 

机构地区：[1]云南省中医中药研究院,昆明650223 [2]大理学院,云南大理671000 [3]云南省红河州建水县中医医院,云南红河654399

出　　处：《中国艾滋病性病》2016年第6期410-412,共3页Chinese Journal of Aids & STD

基　　金：云南省科技计划项目(2013FB095)

摘　　要：目的探讨中医药干预,对艾滋病病毒(HIV)感染者T细胞表面程序性死亡分子1(PD-1)和T细胞免疫球蛋白及黏蛋白结构域分子3(Tim-3)表达水平变化的影响。方法用流式细胞分析术,分别检测新发感染HIV未治疗者、纯中医药治疗2年的HIV感染者、中西医结合治疗2年的HIV感染者的T细胞亚群计数和T细胞表面PD-1和Tim-3表达。结果单纯中医或中西医结合治疗的HIV感染者,其CD4+T淋巴细胞计数>460个/μL,T细胞表面PD-1和Tim-3呈低水平表达;新发感染HIV未治疗者,其CD4+T淋巴细胞计数为507个/μL,T细胞表面PD-1和Tim-3也呈低水平表达,组间比较差异无统计学意义(P>0.05)。结论纯中医药或中西医结合治疗HIV感染,一定时间内可以稳定HIV感染者的CD4+T淋巴细胞计数,维持CD4、CD8细胞表面PD-1和Tim-3的低表达,对延缓病程起到积极作用。Objective To explore T cell surface PD-1and Tim-3expression changes in HIV infected patients with TCM treatment.Methods Flow cytometry was used to test the expression of T cell counts and T cell surface PD-1and Tim-3of the HIV/AIDS patients with an untreated group,a TCM treated group and an integrative medicine treated group for 2years.Results HIV/AIDS patients of TCM treated and integrative medicine treated groups had CD4＋T cell counts above 460cell/μL and the CD4＋T,CD8＋T cell surface expression of PD-1and Tim-3remained low.The untreated HIV positive patients had CD4＋T cell counts above 507cell/μL and CD4＋T,CD8＋T cell surface expression of PD-1and Tim-3kept low too.The difference between groups（P〉0.05）was not statistically significant.Conclusion TCM and integrative medicine treatment can keep CD4＋T cell counts of the HIV/AIDS patients stable for a certain time,and maintain the CD4＋T,CD8＋T cell surface expression of Tim-3and PD-1lower,which play an active role in delaying the disease progression.

关 键 词：艾滋病病毒感染 中医药 程序性死亡分子1 T细胞免疫球蛋白及黏蛋白结构域分子3 

分 类 号：R512.91[医药卫生—内科学] R373.9[医药卫生—临床医学]