血清模式识别受体在重度哮喘患儿中表达的意义  

Roles of pattern recognition receptor in pediatric patients with severe asthma

作　　者：罗丽丹 王恩智[2] 童夏生[3] 罗冬娇[4] 

机构地区：[1]台州市妇幼保健院儿科,浙江省台州317000 [2]台州市中西医结合医院检验科,浙江省台州317523 [3]台州市中西医结合医院儿科,浙江省台州317523 [4]杭州师范大学钱江学院医学科学系,浙江省杭州310012

出　　处：《中国基层医药》2016年第13期1991-1994,共4页Chinese Journal of Primary Medicine and Pharmacy

基　　金：浙江省自然科学基金资助项目（LY13H080006）;浙江省温岭市科技基金资助项目（2013-1-62）

摘　　要：目的：检测重度哮喘患儿血清Toll样受体1（TLR1）、核苷酸结合寡聚域样受体1（NOD1）、Dectin及结合膜蛋白细胞分化抗原14（sCD14）的表达，探讨模式识别受体在哮喘发病机制中的作用。方法标本来自45例重度哮喘患儿（哮喘组），分别在急性期、恢复期采集静脉血，采用酶联免疫吸附试验法检测血清NOD1、TLR1、Dectin 和 sCD14的蛋白浓度，并与30例健康儿童（对照组）进行对比分析。结果哮喘组急性期血清 NOD1、TLR1、Dectin、sCD14蛋白浓度分别为（65．53±19．95）ng／mL、（10．46±3．35）ng／mL、（80．38±19．51）ng／mL、（4．51±1．29）ng／mL，均显著高于对照组[分别为（25．57±9．64）ng／mL、（5．54±1．49）ng／mL、（47．37±15．16）ng／mL、（2．21±0．68）ng／mL]（t ＝11．57、8．63、7．82、9．95，均 P ＜0．01）。哮喘组恢复期血清NOD1、Dectin、sCD14蛋白浓度分别为（39．67±11．25）ng／mL、（60．04±16．76）ng／mL、（3．38±0．97）ng／mL，均显著低于急性期（t ＝8．19、6．32、5．42，均 P ＜0．01）；而 TLR1的蛋白浓度[（9．51±3．19）ng／mL]与急性期差异无统计学意义（t ＝1．27，P ＞0．05）。NOD1与 CD14的表达呈显著直线正相关（r ＝0．49，P ＜0．01）。结论重度哮喘患儿急性期血清模式识别受体 NOD1、TLR1、Dectin 和 sCD14的表达水平升高，恢复期下降，它们可能协同参与了哮喘的发病机制。Objective To investigate the potential roles of pattern recognition receptor in the pathogenesis of asthma,the concentrations of serum nucleotide -binding oligomerization domain like receptors 1 （NOD1 ）and Toll like receptor 1（TLR1）and Dectin and sCD14 protein were determined.Methods Blood samples were obtained from 45 cases of severe asthma（asthma group）during acute attack and convalescent period,the concentrations of serum NOD1,TLR1,Dectin and sCD14 protein were assessed using enzyme linked immunosorbent assay（ELISA）,and compared with 30 healthy children（control group）.Results The concentrations of NOD1,TLR1,Dectin and sCD14 protein were significantly higher in acute attack period of the asthma group[（65.53 ±19.95）ng/mL,（10.46 ±3.35）ng/mL, （80.38 ±19.51）ng/mL,（4.51 ±1.29）ng/mL]than those in the control group[（25.57 ±9.64）ng/mL,（5.54 ± 1.49）ng/mL,（47.37 ±15.16）ng/mL,（2.21 ±0.68）ng/mL]（t =11.57,8.63,7.82,9.95,all P 〈0.01）.Moreo-ver,the concentrations of serum NOD1,Dectin and sCD14 protein were dramatically decreased in convalescent period of the asthma group[（39.67 ±11.25）ng/mL,（60.04 ±16.76）ng/mL,（3.38 ±0.97）ng/mL]compared with acute asthma attack period（t =8.19,6.32,5.42,all P 〈0.01）,while the concentration of serum TLR1 was not differentnbsp;between this two period （9.51 ±3.19）ng/mL（t =1.27,P 〉0.05）.Furthermore,level of NOD1 was positively corre-lated with sCD14（r =0.49,P 〈0.01）.Conclusion Levels of serum NOD1,TLR1,Dectin and sCD14 protein were increased in acute severe asthma,whereas,they were decreased in convalescent period.The elevation of them indicated that pattern recognition reception has synergistic function in the pathogenesis of asthma attack.

关 键 词：哮喘 模式识别受体 发病机制 重度 

分 类 号：R725.6[医药卫生—儿科]