Differential gene expression in porcine SK6 cells infected with wild-type and SAP domain-mutant foot-and-mouth disease virus  

作　　者：Zixin Ni Fan Yang Weijun Cao Xiangle Zhang Ye Jin Ruoqing Mao Xiaoli Du Weiwei Li Jianhong Guo Xiangtao Liu Zixiang Zhu Haixue Zheng 

机构地区：[1]State Key Laboratory of Veterinary Etiological Biology,National Foot and Mouth Diseases Reference Laboratory,Key Laboratory of Animal Virology of Ministry of Agriculture,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences [2]College of Veterinary Medicine,China Agricultural University

出　　处：《Virologica Sinica》2016年第3期249-257,共9页中国病毒学（英文版）

基　　金：supported by grants from the National Science and Technology Ministry (2015BAD12B04);National Natural Sciences Foundation of China (No.31302118,31502042 and 31402179);the Gansu Science Foundation for Distinguished Young Scholars (no.145RJDA328);the International Atomic Energy Agency (16025/R0);the Key technologies R&Dprogram of Gansu Province (1302NKDA027)

摘　　要：Foot-and-mouth disease virus(FMDV) is the causative agent of a highly contagious disease in livestock. The viral proteinaseL^(rop) of FMDV is involved in pathogenicity, and mutation of theL^(rop) SAP domain reduces FMDV pathogenicity in pigs. To determine the gene expression profiles associated with decreased pathogenicity in porcine cells, we performed transcriptome analysis using next-generation sequencing technology and compared differentially expressed genes in SK6 cells infected with FMDV containingL^(rop) with either a wild-type or mutated version of the SAP domain. This analysis yielded 1,853 genes that exhibited a ≥ 2-fold change in expression and was validated by real-time quantitative PCR detection of several differentially expressed genes. Many of the differentially expressed genes correlated with antiviral responses corresponded to genes associated with transcription factors, immune regulation, cytokine production, inflammatory response, and apoptosis. Alterations in gene expression profiles may be responsible for the variations in pathogenicity observed between the two FMDV variants. Our results provided genes of interest for the further study of antiviral pathways and pathogenic mechanisms related to FMDV L^(rop).Foot-and-mouth disease virus（FMDV） is the causative agent of a highly contagious disease in livestock. The viral proteinaseL^（rop） of FMDV is involved in pathogenicity, and mutation of theL^（rop） SAP domain reduces FMDV pathogenicity in pigs. To determine the gene expression profiles associated with decreased pathogenicity in porcine cells, we performed transcriptome analysis using next-generation sequencing technology and compared differentially expressed genes in SK6 cells infected with FMDV containingL^（rop） with either a wild-type or mutated version of the SAP domain. This analysis yielded 1,853 genes that exhibited a ≥ 2-fold change in expression and was validated by real-time quantitative PCR detection of several differentially expressed genes. Many of the differentially expressed genes correlated with antiviral responses corresponded to genes associated with transcription factors, immune regulation, cytokine production, inflammatory response, and apoptosis. Alterations in gene expression profiles may be responsible for the variations in pathogenicity observed between the two FMDV variants. Our results provided genes of interest for the further study of antiviral pathways and pathogenic mechanisms related to FMDV L^（rop）.

关 键 词：Foot-and-mouth disease virus（FMDV） leader protein SAP region transcriptome analysis 

分 类 号：S852.65[农业科学—基础兽医学]