薄层色谱法鉴别三黄片中的有效成分  

The identification of active ingredients three yellow tablets by TLC

作　　者：李蒙蒙[1] 田小杏 马丽娟[2] 蒲晓辉[1] 袁琦[1] 宗兰兰[3] 徐颖峰[4] 

机构地区：[1]河南大学药物研究所,河南开封475004 [2]宁夏医科大学总院儿童医院药剂科,银川750004 [3]河南大学纳米材料工程研究中心,河南开封475004 [4]天津天士力现代中药资源有限公司,天津300402

出　　处：《河南大学学报（医学版）》2016年第2期81-84,109,共5页Journal of Henan University:Medical Science

基　　金：NSFC-河南人才培养联合基金资助项目(U1304826);河南省科技攻关计划项目(152102310077)

摘　　要：〔目的〕探索薄层色谱二次展开同时鉴别三黄片中7种有效成分的方法。〔方法〕参照薄层色谱法,以硅胶G为吸附剂,先以正己烷、乙酸乙酯、甲酸(30∶10∶0.5)为展开剂,分离三黄片中大黄的5种成分(大黄酚、大黄素甲醚、大黄素、大黄酸、芦荟大黄素),再以乙酸乙酯、丁酮、甲酸、水(10∶7∶1∶1)为展开剂,进行二次展开,分离小檗碱和黄芩苷。分别在波长为365 nm和254 nm的紫外灯下检视。最后分别对三个不同厂家的各三批样品进行了鉴别。〔结果〕在三黄片供试品溶液的色谱图中可以观察到,三黄片供试品与对照品色谱在相同位置上呈相同颜色的斑点。〔结论〕通过实验可知,依本实验方案所得薄层色谱图,斑点边缘清晰、重现性好、专属性强、操作简单,可用于三黄片7种有效成分的鉴别。〔Objective〕To explore the method of secondary separation of the TLC and to identify the seven kinds of effective components in Sanhuang tablets.〔Methods〕To explore the method of secondary separation of the TLC and to identify the seven kinds of effective components in Sanhuang tablets.〔Results〕The testing solution of Sanhuang tablet and the reference substance both showed the same color spots in the same position by TLC.〔Conclusion〕The experiments indicate： According to the image of TLC, the TLC is Spot sharp edges, reliable and specific method with good reproducilility, to be used identification for the seven kinds of effective components in Sanhuang tablets.

关 键 词：三黄片 成分鉴定 薄层色谱法 

分 类 号：TQ460.72[医药卫生—药物分析学]