南方、花生和爪哇根结线虫PCR检测方法  

Detection of Meloidogyne incognita,M. arenaria and M. javanica using PCR method

作　　者：容万韬 王金成[2] 林宇[2] 顾建锋[3] 陈先锋[3] 魏亚东[2] 

机构地区：[1]河池学院,广西河池546300 [2]天津出入境检验检疫局动植物与食品检测中心 [3]宁波出入境检验检疫局技术中心

出　　处：《植物检疫》2016年第3期42-47,共6页Plant Quarantine

基　　金：国家质检总局课题(2011IK176);国家科技支撑计划(2012BAK11B03);宁波市科技创新团队(2015C110018);天津出入境检验检疫局科技项目(TK072-2013)

摘　　要：为快速、准确、稳定的鉴定南方、花生和爪哇根结线虫,利用已报道的南方和爪哇根结线虫的两对特异性引物,结合本研究设计的花生根结线虫特异性引物,通过优化PCR反应体系,建立了3种根结线虫的PCR检测方法。结果表明,该方法能够特异性扩增以上3种根结线虫,特征片段长度分别为399、335和670 bp,灵敏度达到单条2龄幼虫的水平。研究结果将为以上3种根结线虫的快速鉴定提供技术支持。To identify Meloidogyne incognita,M. arenaria and M. javanica with rapid,accurate and stable method,two pairs of specific primers for M. javanica and M. incognita from previous studies were chosen,combined with a pair of specific primers for M. arenaria designed in this study,a PCR method was constructed by optimizing PCR reaction parameters. The results showed that the above three Meloidogyne species could be identified with the present method,with characteristic amplicon length of 399,335 and670 bp for M. incognita,M. arenaria and M. javanica,respectively. The sensitivity of this method is one single second-stage juvenile. Results of this study will provide techinical support for the identification of three above-mentioned root-knot nematodes.

关 键 词：根结线虫 南方根结线虫 花生根结线虫 爪哇根结线虫 PCR 2龄幼虫 分子鉴定 

分 类 号：S432.45[农业科学—植物病理学]