螺内酯对心肌肥厚大鼠心肌组织中可溶性ST2表达的影响  

Effects of spironolactone on soluble ST2 in myocardial tissue of hypertrophy rats

作　　者：邢瑞星 赵玉兰[1] 黄亚萍[1] 董静[1] 

机构地区：[1]郑州大学第二附属医院心内科,郑州450014

出　　处：《郑州大学学报（医学版）》2016年第3期393-396,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:研究螺内酯对心肌肥厚大鼠IL-33/ST2通路中可溶性ST2(s ST2)的影响。方法:选取健康雄性SD大鼠30只,随机分为对照组(n=10)、模型组(n=10)、药物干预组(n=10)。对照组皮下注射生理盐水,模型组皮下注射异丙肾上腺素(ISO),药物干预组皮下注射ISO+螺内酯20 mg/(kg·d)灌胃,余各组用等量生理盐水灌胃。14 d后处死大鼠,测量心脏质量指数、左心室质量指数及心肌细胞直径;免疫组化法及RT-PCR法检测心肌组织中s ST2的表达。结果:模型组与药物干预组心脏质量指数、左心室质量指数及心肌细胞直径均高于对照组(P<0.05);模型组s ST2的表达高于对照组和药物干预组(P<0.05)。结论:螺内酯可能通过调节IL-33/ST2通路中s ST2的表达,抑制ISO诱导的心肌肥厚。Aim： To study the effects of spironolactone on s ST2 of IL-33/ST2 pathway in myocardial hypertrophy rats.Methods： A total of 30 healthy male SD rats were randomly allocated into control group（ n = 10）,model group（ n = 10） and drug intervention group（ n = 10）. LVM/BM,HM/BM and the size of cardiomyocyte were detected. s ST2 mRNA and protein in left ventricular tissue were analyzed by RT-PCR and immunohistochemistry. Results： LVM/BM and HM/BM in the drug intervention group and model group were higher than those in control group（ P〈0. 05）. The expression of s ST2 in the model group was higher than that in the control group and drug intervention group（ P〈0. 05）. Conclusion： Spironolactone could modulate the expression of s ST2 in IL-33/ST2 pathway,and alleviate myocardial hypertrophy caused by isoproterenol.

关 键 词：螺内酯 心肌肥厚 可溶性ST2 大鼠 

分 类 号：R54[医药卫生—心血管疾病]