Effect of moxa-burning heat stimulating Liangmen(ST 21) and Zusanli(ST 36) on proliferation and apoptosis signaling proteins in rats with stress-induced gastric ulcer  

作　　者：Peng Li Wang Yadong Chang Xiaorong Wu Huangan Liu Mi Ma Fuqiang Wang Hong Chen Jiaolong Wang Chao Quan Renfu Yang Zongbao 

机构地区：[1]Medical College, Xiamen University [2]Acupuncture and Moxibustion college, Hunan University of TCM [3]Research Institute of Acupuncture and Moxibustion, Shanghai University of TCM [4]Research Institute of Acupuncture and Moxibustion, Xiaoshan Traditional Chinese Medical Hospital

出　　处：《Journal of Traditional Chinese Medicine》2016年第3期340-346,共7页中医杂志（英文版）

基　　金：National Natural Science Foundation of China:To Study the Pre-protection Mechanism of Moxibustion Treatment of Acupoints of Stomach Meridian Regulating Egfr Signaling Pathway in Chronic Atrophic Gastritis Precancerous Lesions(No.81260556);National Natural Science Foundation of China:to Study Brain-stomach Response Mechanism of Electro-acupuncture Treatment of Acupoints of Stomach Meridian Repairing Gastric Mucosa Damage Based on Nuclear Magnetic Resonance Metabonomics Technology(No.81473751);the National Key Basic Research Development Plan(973 Plan):the Basic and Clinical Research of Moxibustion and Acupuncture Effect Characteristics and the Similarities and Differences of Their Effect Response(2015cb554502);Scientific Research Foundation for the Returned Overseas Chinese Scholars,Ministry of Education of China:to Study the Mechanism of Compatibility of Yiqi And Huoxue Traditional Chinese Herbs Selective Amplifying Regulatory T Cells to Inhibit Atherosclerosis(No.2015-311)

摘　　要：OBJECTIVE: To observe the effect of moxa-burning heat stimulating acupoints of Liangmen(ST 21)and Zusanli(ST 36) on the proliferation and apoptosis signaling proteins in rats with stress-induced gastric ulcer.METHODS: Forty rats were randomly divided into four groups: negative control(NC), ulcer control(UC), acupoints of stomach meridian(ASM), and acupoints control(AC). The acute gastric ulcer model was established by bound and water immersion.Rats in NC and UC groups didn't receive any moxa-burning heat stimulating treatment, while rats in ASM and AC groups were treated with buringmoxa heat stimulating the acupoints of Liangmen(ST 21) and Zusanli(ST 36) and their controlled points, respectively. Rats in all groups were sacrificed after 12 consecutive days treatment. The ulcer index was evaluated by using Guth's method.The expression of tumor necrosis factor-alpha(TNF-α), apoptotic protease activating facter-1(Apaf-1), Caspase-3, p21 activated kinase 1(PAK1),extracellular regulated protein kinases 2(ERK2),phosphorylated ERK2(p ERK2), phosphoinositide3-kinase(PI3K) and RAC-alpha serine/threonine-protein kinase(Akt) in gastric mucosa was detected by enzyme linked immunosorbent assay(ELISA).RESULTS: Compared with UC group, the ulcer index of ASM and AC groups decreased, and the injured gastric mucosa was improved, the expression of TNF-α, Apaf-1 and Caspase-3 in gastric mucosa was significantly reduced(P < 0.05), while the ex-pression of PAK1, ERK2, p ERK2, PI3 K and Akt in gastric mucosa was significantly increased(P < 0.05).And ASM showed better effect than AC group(P <0.05).CONCLUSION: Moxa-burning Heat stimulating of Liangmen(ST 21) and Zusanli(ST 36) could promote the recovery of gastric mucosal lesion probably by inhibiting cell apoptosis and promoting cell proliferation in stress-induced gastric ulcer.OBJECTIVE： To observe the effect of moxa-burning heat stimulating acupoints of Liangmen（ST 21）and Zusanli（ST 36） on the proliferation and apoptosis signaling proteins in rats with stress-induced gastric ulcer.METHODS： Forty rats were randomly divided into four groups： negative control（NC）, ulcer control（UC）, acupoints of stomach meridian（ASM）, and acupoints control（AC）. The acute gastric ulcer model was established by bound and water immersion.Rats in NC and UC groups didn＇t receive any moxa-burning heat stimulating treatment, while rats in ASM and AC groups were treated with buringmoxa heat stimulating the acupoints of Liangmen（ST 21） and Zusanli（ST 36） and their controlled points, respectively. Rats in all groups were sacrificed after 12 consecutive days treatment. The ulcer index was evaluated by using Guth＇s method.The expression of tumor necrosis factor-alpha（TNF-α）, apoptotic protease activating facter-1（Apaf-1）, Caspase-3, p21 activated kinase 1（PAK1）,extracellular regulated protein kinases 2（ERK2）,phosphorylated ERK2（p ERK2）, phosphoinositide3-kinase（PI3K） and RAC-alpha serine/threonine-protein kinase（Akt） in gastric mucosa was detected by enzyme linked immunosorbent assay（ELISA）.RESULTS： Compared with UC group, the ulcer index of ASM and AC groups decreased, and the injured gastric mucosa was improved, the expression of TNF-α, Apaf-1 and Caspase-3 in gastric mucosa was significantly reduced（P 0.05）, while the ex-pression of PAK1, ERK2, p ERK2, PI3 K and Akt in gastric mucosa was significantly increased（P 0.05）.And ASM showed better effect than AC group（P 0.05）.CONCLUSION： Moxa-burning Heat stimulating of Liangmen（ST 21） and Zusanli（ST 36） could promote the recovery of gastric mucosal lesion probably by inhibiting cell apoptosis and promoting cell proliferation in stress-induced gastric ulcer.

关 键 词：MOXIBUSTION Point ST 21（Liangmen） Point ST 36（Zusanli） Stomach ulcer Apoptosis Cell proliferation 

分 类 号：R245[医药卫生—针灸推拿学]