壮医药线点灸疗法对寒湿凝滞型痛经大鼠NO、ET-1的影响  

Effect of Zhuang's herbal moxibustion on the expression of nitric oxide,endothelin-1 in rats with primary dysmenorrheal

作　　者：胡雷蕾 周宇博[1] 池碧霞 吴红斌[2] 

机构地区：[1]桂林医学院,广西桂林541004 [2]桂林医学院附属医院中医科,广西桂林541001

出　　处：《华夏医学》2016年第2期5-9,共5页Acta Medicinae Sinica

摘　　要：目的:观察壮医药线点灸疗法对痛经模型大鼠一氧化氮(NO)、内皮素1(ET-1)的影响,探讨其治疗效应的可能机制。方法:100只雌性SD大鼠随机分为正常对照组(A)、模型组(B)、壮医药线点灸组(C)、月月舒组(D)、针刺组(E),C组、D组、E组持续干预1周,比较各组大鼠的扭体反应潜伏期和30 min扭体次数,并分别检测大鼠血浆和子宫匀浆中NO、ET-1的表达水平。结果:(1)A组无扭体,与B组相比,C、D、E组首次扭体潜伏期明显延长,30 min扭体次数减少(P<0.01);C、D、E组扭体次数差异无统计学意义(P>0.05),但与D组相比,C组扭体潜伏期延长(P<0.01),且3组中C组扭体抑制率最高。(2)与A组比较,C组、D组、E组大鼠NO、ET-1含量无统计学意义(P>0.05);与B组比较,C组、D组、E组ET-1值显著降低,NO显著增加(P<0.01);C、D、E 3组间NO、ET-1值比较无统计学意义(P>0.05)。结论:壮医药线点灸疗法治疗寒湿凝滞型痛经的作用机制可能与增加大鼠NO水平、降低ET-1水平有关。Objective： To observe the effect of Zhuang＇s herbal moxibustion on the expression of NO,ET-1in model rats with primary dysmenorrheal,and investigate the possible mechanism of Zhuang＇s herbal moxibustion in the treatment of the dysmenorrheal model. Methods： 100 female rats were randomly assigned to blank group（Group A）, model group（Group B）, Zhuang＇s herbal moxibustion group（Group C）,Yueyueshu granule group（Group D）, acupuncture group（Group E）. After received one week of administration,the latency period and the incidence of writhing body within 30 min were observed in each group and the serum levels of NO and ET-1 were detected. Results：（1） There were none incidence of writhing body in Group A. Compared with Group B, the latency period of writhing body was longer and the incidence of writhing body was less in C, D, E Group（P 0.01）. There was no significant difference between Group C, D and E in the incidence rate of writhing body（P 0.05）. But compared with Group D, the latency period ofwrithing body was longer in C Group（ P 0.01）. And in C, D, E Group, the control rate of writhing body was highest in Group C.（2）Compared with Group A, there was no significant difference between Group C,D and E in the serum and uterine tissues levels of NO and ET-1（P 0.05）. Compared with Group B, the serum and uterine tissues level of NO was increased, and ET-1 was obviously decreased（ P 0.01）. There was no significant difference between Group C,D and E in the serum and uterine tissues levels of NO, ET-1（P 0.05）. Conclusion ： The efficacy of Zhuang＇s herbal moxibustion on primary dysmenorrheal might be associated with regulating the low level of ET-1 and high level of NO in serum.

关 键 词：壮医药线点灸 寒湿凝滞型原发性痛经 一氧化氮 内皮素 痛经大鼠模型 

分 类 号：R271.113[医药卫生—中医妇科学]