基因芯片检测临床标本结核分枝杆菌异烟肼和利福平耐药性的研究  

Study of gene chip in detecting clinical samples Mycobacterium tuberculosis resistance to Isoniazid and Rifampicin

作　　者：熊敏[1] 骆科文[1] 周忠[1] 欧维正[1] 

机构地区：[1]贵阳市肺科医院结核科,贵州贵阳550003

出　　处：《华夏医学》2016年第2期48-51,共4页Acta Medicinae Sinica

摘　　要：目的:评价基因芯片检测技术在结核病诊断中的效果。方法:收集我院2012年4月至2013年6月符合标准的432例进行基因芯片耐药基因技术检测,同时进行传统比例法药物敏感试验,后者作为金标准,对两组检测结果进行比较评价。结果:基因芯片技术检测结核菌异烟肼耐药性的灵敏度和特异度分别为78.13%和89.88%,与金标准差异有统计学意义(P<0.01);基因芯片技术检测结核菌利福平耐药性的灵敏度和特异度分别为86.57%和91.95%,与金标准差异有统计学意义(P<0.01);基因芯片技术检测结核菌同时耐异烟肼和利福平(耐多药)的灵敏度和特异度分别为70.11%和90.14%,与金标准差异有统计学意义(P<0.01)。结论:基因芯片检测技术是一种快捷、简便、灵敏度高的方法,对于结核菌耐药性的检测具有很好的效果,但直接用于临床标本检测结核菌的耐药性不够理想。Objective：To evaluate the diagnosis effect of gene chip in detecting Mycobacterium tuberculosis.Methods： 432 specimens of patients with tuberculosis in hospital and clinic were examined for the resistance by gene chip, and the drug sensitivity test of traditional proportion methods. Meanwhile the drug sensitivity test, as a golden standard were compared with the resistance by gene clip. Results： Their sensitivity and specificity were separately 78.13% and 89.88% with detecting Mycobacterium tubercuiosis resistance to Isoniazied and the golden standard, there were markedly statistial difference between the two groups（P 0.01）. Their sensitivity and specificity were separately 86.57% and 91.95% with detecting Mycobacterium tubercuiosis resistance to Rifampicin and the golden standard, there were markedly statistial difference between the two groups（P 0.01）. Their sensitivity and specificity were separately 70.11% and90.14% with detecting Mycobacterium tubercuiosis resistance to Isoniazid and Rifampicin and the golden standard, there were markedly statistial difference between the two groups（P0.01）. Conclusion： Gene chip detecting is a rapid, simply and high sensitive way, and it is very good reality for detecting Mycobacterium tuberculosis resistance, but it is not better ideal to detect Mycobacterium tuberculosis resistance in clinical specimens directly.

关 键 词：基因芯片 结核分枝杆菌 耐药性 

分 类 号：R978.3[医药卫生—药品]