黄酒多酚对Hcy诱导的血管平滑肌细胞MMP-2/9表达与活性的影响  

Yellow wine polyphenol compounds inhibit homocysteine-induced expression of MMP-2/9 in vascular smooth muscle cells through inhibiting PI3K/AKT pathway

作　　者：刘龙斌[1] 孟立平[1] 季政[1] 许富康[1] 池菊芳[1] 郭航远[1] 

机构地区：[1]绍兴市人民医院心内科,312000

出　　处：《浙江医学》2016年第12期911-914,935,共5页Zhejiang Medical Journal

基　　金：浙江省自然科学基金项目(LY14H020002)

摘　　要：目的：探讨黄酒多酚（YWPC）对同型半胱氨酸（Hcy）诱导的大鼠血管平滑肌细胞（VSMCs）基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶-9（MMP-9）表达和活性的影响及其信号通路。方法 SD大鼠主动脉VSMCs原代细胞培养鉴定，取4~7代细胞用于实验。YWPC组VSMCs分别用1、10、100mg/L的YWPC干预，采用 Western blot法检测VSMCs中MMP-2、MMP-9、AKT、pAKT的表达情况；明胶酶谱检测VSMCs中MMP-2和MMP-9的活性。用IGF-1（3ng/ml）干预VSMCs 8 h激活PI3K/AKT通路后，用YWPC干预VSMCs，检测MMP-2、MMP-9的表达和活性。结果 Western blot结果显示，与Hcy组比较， YWPC组VSMCs中MMP-2/9的表达减少，pAKT表达减少（均P＜0.01）；明胶酶谱结果显示，与Hcy组比较，YWPC组VSMCs中MMP-2/9的活性降低。在加入IGF-1后,与对照组比较，IGF-1组VSMCs中pAKT、MMP-2/9的表达和活性增加（P＜0.01）；与YWPC组比较，YWPC＋IGF-1组VSMCs中pAKT、MMP-2/9的表达和活性增加（P＜0.01）。结论 YWPC通过抑制PI3K/AKT信号通路抑制Hcy诱导的VSMCs中MMP-2/9的表达和活性。Objective To investigate the effect of yel ow wine polyphenol compounds（YWPC） on expression of MMP- 2/9 in rat aortic vascular smooth muscle cel s （VSMCs） induced by homocysteine （Hcy） and its mechanism. Methods The primarily cultured VSMCs were incubated with 500μmol/L Hcy for 12h; then VSMCs were treated with YWPCs in concentration of 1mg/L, 10mg/L or 100mg/L, respectively. IGF- 1 （3ng/ml） was used to activate PI3K/AKT pathway. The expressions of MMP- 2, MMP- 9, AKT, pAKT in VSMCs were detected with Western blotting; the activity of MMP- 2/9 was examined with gelatin zymography. Results Western blotting showed that compared with Hcy group, the expressions of MMP- 2/9, pAKT in YWPC groups were decreased. Gelatin zymography demonstrated that compared with Hcy group, the activity of MMP- 2/9 was decreased in the YWPC groups. Compared with control group, the expressions of pAKT, MMP- 2/9 was increased in the IGF- 1 group （P〈0.01）. Compared with YWPC groups the expression of pAKT, MMP- 2/9 was increased in YWPC＋IGF- 1 group （P〈0.01）. Conclusion YWPC inhibits Hcy- induced expression of MMP- 2/9 in VSMCs by suppressing PI3K/AKT signaling pathway.

关 键 词：血管平滑肌细胞 黄酒多酚 基质金属蛋白酶 

分 类 号：R54[医药卫生—心血管疾病]