丹蒌片和血府逐瘀颗粒对动脉粥样硬化大鼠脂质代谢和炎症反应影响的比较研究  

Comparison of lipid metabolism and inflammatory reaction in atherosclerotic rats receiving treatment with Danlou tablets or Xuefu Zhuyu granules

作　　者：陈洁[1] 缪静[2] 徐晓明[1] 戴金[1] 毛威[1] 

机构地区：[1]浙江中医药大学附属第一医院心内科,310006 [2]浙江中医药大学第一临床医学院

出　　处：《心电与循环》2016年第3期181-185,共5页Journal of Electrocardiology and Circulation

基　　金：浙江省中医药管理局基金项目(2014ZB043);吴阶平基金项目(320.6750.12397)

摘　　要：目的观察丹蒌片和血府逐瘀颗粒对动脉粥样硬化大鼠脂质代谢和炎症反应的影响。方法 40只雄性Wistar大鼠随机分为正常组、模型组、阿托伐他汀钙组、丹蒌片组、血府逐瘀颗粒组,每组8只。正常组大鼠喂食基础饲料,余4组均采用高脂饮食联合维生素D_3腹腔注射的方法复制大鼠动脉粥样硬化模型。模型组、阿托伐他汀钙组、丹蒌片组、血府逐瘀颗粒组分别给予生理盐水、阿托伐他汀钙混悬液、丹蒌片混悬液、血府逐瘀颗粒混悬液灌胃干预8周。干预结束后,检测大鼠血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL—C)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF—α)、单核细胞趋化蛋白-1(MCP-1)、氧化低密度脂蛋白(ox—LDL)、脂蛋白相关磷脂酶A2(LP—PLA2)水平,采用荧光定量PCR法检测大鼠胸主动脉内IL-6、TNF—α、MCP-1、LP—PLA2 mRNA表达。结果与正常组比较,模型组大鼠血清中TC、TG、LDL—C和IL-6、TNF—α、MCP-1、ox—LDL、LP—PLA2水平升高(均P<0.05),胸主动脉内炎症因子IL-6、TNF—α、MCP-1和LP—PLA2 mRNA表达上调(均P<0.05)。与模型组比较,阿托伐他汀钙组与丹蒌片组均能降低动脉粥样硬化模型大鼠血清中TC、TG、LDL—C和IL-6、TNF—α、MCP-1、ox—LDL、LP—PLA2水平(均P<0.05),胸主动脉内IL-6、TNF—α、MCP-1和LP—PLA2 mRNA表达下调(P<0.05),组间比较差异无统计学意义(均P>0.05)。血府逐瘀颗粒组能降低血清中TC和炎症因子IL-6、TNF—α、MCP-1,抑制胸主动脉内IL-6、TNF—α、MCP-1 mRNA表达(均P<0.05),而血清中的TG、LDL—C、ox—LDL、LP—PLA2水平和胸主动脉内LP—PLA2 mRNA表达与模型组相比差异无统计学意义(均P>0.05)。结论丹蒌片和血府逐瘀颗粒均能抑制动脉粥样硬化大鼠炎症因子的表达,丹蒌片调节脂质代谢的作用优于血府逐瘀颗粒。Objective To investigate the effects of Chinese medicine Danlou tablets and Xuefu Zhuyu granules on blood lipid metabolism and inflammatory reaction in rat model of atherosclerosis. Methods Forty male Wistar rats were randomly divided into five groups, namely normal control group, model control group, atorvastatin group, Danlou group and Xuefu Zhuyu group. Normal control group was given basic forage, while other four groups were given high fat forage plus intraperitoneal injection of vitamin D3 to establish atherosclerosis model, and then received intragastric administration of saline, atorvastatin suspension, Danlou tablet suspension and Xuefu Zhuyu granule suspension for 8 wk, respectively. After intervention, serum lipid and IL- 6, TNF- α, MCP- 1, ox- LDL, LP- PLA2 levels were measured by ELISA method, the expression of IL- 6, TNF- α, MCP- 1, LP- PLA2 mRNAs in thoracic aorta were detected by real- time fluorescent quantitative PCR （RT- fqPCR）. Results Compared with normal control group, serum TC, TG, LDL- C, IL- 6, TNF- α, MCP- 1, ox- LDL and LP- PLA2 levels and the expressions of IL- 6, TNF- α, MCP- 1 and LP- PLA2 mRNAs in thoracic aorta of model group were significantly increased （al P〈0.05）. Compared with model control group, serum TC, TG, LDL- C, IL- 6, TNF- α, MCP- 1, ox- LDL and LP- PLA2 levels and the expressions of IL- 6, TNF- α, MCP- 1 and LP- PLA2 mRNAs in thoracic aorta of atorvastatin group and Danlou group were significantly decreased （al P〈0.05）;and there were no significant differences between atorvastatin group and Danlou group （P 〉0.05）. Serum TC, IL- 6, TNF- α, MCP- 1 levels and expressions of IL- 6, TNF- α, MCP- 1 mRNA in thoracic aorta of Xuefu Zhuyu group were significant decreased （al P〈0.05） compared with model control group, while there were no significant differences in serum TG, LDL- C, ox- LDL, LP- PLA2 levels and expression of LP- PLA2 mRNA in thoracic aorta between two groups （P〉0.05）. Conclusion Both Danlou tablets and Xuefu Zhuyu granules

关 键 词：动脉粥样硬化 炎症反应 脂质代谢 丹蒌片 血府逐瘀颗粒 

分 类 号：R285.5[医药卫生—中药学]