消岩汤对环磷酰胺诱导Lewis肺癌小鼠骨髓抑制的造血微环境的改善研究  

Improvement of Xiaoyan Decoction on hemopoietic microenvironment in Lewis lung cancer mice with myelosuppression induced by cyclophosphamide

作　　者：杨佩颖[1] 李小江[1] 刘宏根[1] 张蕴超[1] 张欣[1] 孔凡铭[1] 贾英杰[1] 

机构地区：[1]天津中医药大学第一附属医院,天津300381

出　　处：《现代药物与临床》2016年第6期747-751,共5页Drugs & Clinic

基　　金：国家自然科学基金资助项目(81273937);天津市应用基础与前沿技术研究计划项目(13JCQNJC10900);天津市科技计划项目(12ZCDZSY15800)

摘　　要：目的观察消岩汤对环磷酰胺诱导Lewis肺癌小鼠造血微环境的影响,探究消岩汤对Lewis肺癌小鼠骨髓抑制的造血微环境的改善作用及其作用机制。方法 SPF级Lewis肺癌小鼠随机分为对照组、模型组、重组人粒细胞刺激因子组和消岩汤组,每组各10只。对照组和模型组ig 0.02 mL/g生理盐水。人粒细胞刺激因子组在造模后sc重组人粒细胞刺激因子注射液15 ng/g,1次/d,连续给药7 d。消岩汤组在造模后ig 2.9 g/mL消岩汤,2次/d,0.2 mL/次,连续给药7 d。采用流式细胞仪分析细胞周期,通过细胞培养技术检测基质细胞分化能力,采用QRT-PCR法检测骨髓细胞Bcl-2和Bax mRNA的基因表达。结果与模型组比较,消岩汤能促进骨髓细胞从G_0/G_1期向S、G2/M期转化(P<0.05);能一定程度上缓解化疗导致的成纤维细胞集落生成单位(CFU-F)形成减少(P<0.05);能上调抗凋亡基因Bcl-2 mRNA的表达((P<0.05)。结论消岩汤能够促进骨髓细胞向增殖周期转化,上调Bcl-2 mRNA表达,改善造血微环境,从而缓解Lewis肺癌骨髓抑制对小鼠造血功能的影响。Objective To observe the effects of Xiaoyan Decoction on hemopoietic microenvironment in Lewis lung cancer mice with myelosuppression induced by cyclophosphamide, and discuss the improvement on hemopoietic microenvironment in Lewis lung cancer mice with myelosuppression and to explore its mechanism. Methods SPF Lewis lung cancer mice were randomly divided into control, model, recombinant human granulocyte colony-stimulating factor（rhG-CSF）, and Xiaoyan Decoction groups, and each group had 10 mice. Mice in the control and model groups were ig normal saline 0.02 mL/g. Mice in the rhG-CSF group were sc Recombinant Human Granulocyte Colony-Stimulating Factor Injection 15 ng/g after be established myelosuppression models, once daily, and be treated for 7 d. Mice in the Xiaoyan Decoction group were ig Xiaoyan Decoction 2.9 g/mL afterbe established myelosuppression models, twice daily, 0.2 mL/time, and be treated for 7 d. The cell cycles were analyzed by flow cytometry, differentiation of stromal cell were determined by cell culture technique, and express levels on Bcl-2 mRNA and Bax mRNA of bone marrow cells were checked by q RT-PCR method. Results Compared with the model group, Xiaoyan decoction could promote the bone marrow cells from G_0/G_1 phase to S and G2 /M phase transformation in the cell cycle（P〈 0.05）, could inhibit the reduction of fibroblast colony forming unit（CFU-F） formation induced by chemotherapy to a certain extent（P〈 0.05）, and could up-regulate the expression of anti apoptotic gene Bcl-2 mRNA（P〈 0.05）. Conclusion Xiaoyan Decoction can promote the transformation of bone marrow cells to proliferation cycles, up-regulate the expression of Bcl-2 mRNA gene, improve hemopoietic microenvironment, and relieve the effect of hematopoietic function in Lewis lung cancer mice with myelosuppression.

关 键 词：消岩汤 LEWIS肺癌 骨髓抑制 造血微环境 基因表达 

分 类 号：R285.5[医药卫生—中药学] R286.3[医药卫生—中医学]