HPLC-ELSD法测定胆南星中胆酸类成分的含量  

Determination of cholic acid constituents from Arisaema cum Bile by HPLC-ELSD

作　　者：蔡宇忆 叶永浩[1] 杨丽莹[1] 黄玉梅 吴志坚 马永德 李书渊[1] 

机构地区：[1]广东药科大学中药学院,广东广州510006 [2]广州市药材公司中药饮片厂,广东广州510360

出　　处：《广东药学院学报》2016年第3期311-314,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：广州市荔湾区科技计划(20133115008)

摘　　要：目的建立HPLC-ELSD法测定胆南星中4种胆酸类成分质量分数的方法。方法色谱柱为WELCH AQ-C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.2%(体积分数)冰醋酸(体积比60∶40),柱温为30℃,流速为1.0 m L/min,检测器为ELSD,载气流速为2.2 m L/min,漂移管温度为90℃。结果胆酸、猪去氧胆酸、鹅去氧胆酸、去氧胆酸均在定量范围内呈良好的线性关系,平均回收率分别为97.62%、98.38%、97.32%、97.86%。结论本方法操作简单,测定结果准确,可用于胆南星中胆酸类成分的质量分数测定及质量控制。Objective To develop a HPLC-ELSD method for the determination of cholic acids from Arisaema eum Bile. Methods WELCH AQ-Cls column （4.6 mm×250 mm, 5 μm）was employed and eluted at 30 ℃ with a mobile phase consisting of aeetonitrile-0.2% glacial acetic acid （ 60 ： 40）, and the flow rate was 1.0 mL/min. The drift-tube temperature of ELSD was 90 ℃, and the flow rate of air was 2.2 mL/min. Results Cholie acid, hyodeoxycholic acid, ehenodeoxyeholic acid and deoxidation cholic acid had good linearity within the quantitative range, and their mean recovery ratios were 97.62%, 98.38%, 97.32% and 97.86%, respectively. Conclusion The method was simple and accurate, which could be used for the determination and quality control of cholic acid constituents from Arisaema cum Bile.

关 键 词：胆南星 HPLC-ELSD法 胆酸 猪去氧胆酸 鹅去氧胆酸 去氧胆酸 

分 类 号：R284.1[医药卫生—中药学]