听觉传导通路下丘区域特异性蛋白分析  

Analysis of inferior colliculus region typical proteins in auditory pathway

作　　者：李宛桐 徐莹 李晓璐 

机构地区：[1]南京医科大学第一附属医院,江苏省人民医院耳鼻咽喉科,WHO预防聋和听力减退合作中心,江苏南京210029

出　　处：《中国耳鼻咽喉头颈外科》2016年第6期331-334,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：江苏省“科教兴卫工程”医学重点人才(RC2011073);江苏省科技厅国际合作项目(BZ2011043);江苏省人力资源和社会保障厅“六大人才高峰”(2009075)联合资助

摘　　要：目的研究听觉传导通路下丘(inferior colliculus,IC)区域特异性蛋白,探索IC在听觉信号整合及处理中的可能作用,探索中枢听觉处理障碍(central auditory processing disorders,CAPD)的潜在致病因素。方法本实验采用同位素标记相对和绝对定量(isobaric tags for relative and absolute quantitation,iTRAQ)方法对出生后60 d雄性SD大鼠IC、耳蜗核(cochlear neucleus,CN)、上橄榄核复合体(superior oliver complex,SOC)、Rest这4个核团胞膜蛋白质组学进行定量和定性分析,得出IC区域特异性蛋白。结果本实验最终共检测IC区域特异性蛋白53种。通过GO富集分析及KEGG通路富集分析,IC区域特异性蛋白主要作用为转运及蛋白定位,调节神经元生长发育和信息整合。结论 53种IC区域特异性蛋白中,Ca MKII蛋白和SV2A蛋白参与维持IC兴奋性-抑制性神经递质平衡,此平衡是听觉信号整合及编码精确性的关键,有助于研究CAPD发病机制。OBJECTIVE To study the proteome of inferior colliculus and determinate the region-typical proteins which may be the candidate cause of the Central Auditory Processing Disorders.METHODS The telencephalon was taken as reference,and then identified and quantified the proteome of IC of adult rats with iTRAQ.Those with higher abundance in inferior colliculus than the other three regions were considered as IC-Region typical proteins,which may lead to functional specializations.RESULTS We identified 1937 cytomembrane proteins in total,among which there are 53 IC-Region typical proteins,which may lead to functional specializations of inferior colliculus.We used GO and KEGG pathway to analyze these proteins and then found that these proteins mainly take part in the regulation of neurons development and information integrations.CONCLUSION Our quantitative comparison of inferior colliculus has revealed two candidate proteins,including CaMKII and SV2 A,which may play important roles in maintaining the balance of excitatory and inhibitory transmitters release.These proteins may be the candidate proteins for Central Auditory Processing Disorders.

关 键 词：蛋白质组学 下丘 中枢听觉处理 同位素标记相对和绝对定量 

分 类 号：R764[医药卫生—耳鼻咽喉科]