盐胁迫下旱地棉叶片数字化基因表达谱分析  

Digital gene expression profile of Gossypium aridum leaves under salt stress

作　　者：翟彩娇 徐鹏[2] 范昕琦[2] 郭琪[2] 张香桂[2] 徐珍珍[2] 沈新莲[2] 

机构地区：[1]南京农业大学,江苏南京210095 [2]江苏省农业科学院经济作物研究所/农业部长江下游棉花油菜重点实验室,江苏南京210014

出　　处：《江苏农业学报》2016年第3期481-489,共9页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31471545);国家转基因生物新品种培育重大专项(2014ZX08005-004-002);江苏省农业自主创新基金项目[CX(14)2065]

摘　　要：为了深入研究棉花耐盐的分子机理,利用数字化基因表达谱技术,分析了盐胁迫处理3 h和72 h时旱地棉叶片中基因表达差异,分别获得1 778(1 046个上调、732个下调)和2 873(2 065个上调、808个下调)个差异表达基因。功能注释分析结果表明,这些差异表达基因主要参与了信号转导、逆境应答、能量代谢和转录调节等方面。随机挑选10个差异表达基因进行qRT-PCR验证,结果表明,qRT-PCR结果与数字化基因表达谱分析结果基本一致。To uncover the salt tolerance mechanisms of Gossypium aridum and improve salinity resistance,the technique of digital gene expression profile was involved to analyze the variations of gene expression in the leaves of upland cotton exposed to 200 mmol/L Na Cl for 3 h and 72 h,respectively. There were 1 778（ 1 046 up-regulated/732 down-regulated） and 2 873（ 2 065 up-regulated/808down-regulated） differentially expressed genes,respectively. Functional annotation analysis revealed that the genes were involved in various metabolic pathways including signal transduction,stress response,energy metabolism,and transcriptional regulation. A total of 10 differentially expressed genes were randomly selected for qRT-PCR validation,and the results were basically consistent with those by the digital gene expression profile.

关 键 词：旱地棉 盐胁迫 数字化表达谱分析 

分 类 号：S562[农业科学—作物学]