灰霉菌毒素诱导番茄抗性突变体及相关防御酶活性  

Resistant mutant induced by toxin of Botrytis cinerea in tomato and the activities of related defensive enzymes

作　　者：赵丽萍[1] 李永灿[1] 赵统敏[1] 陈怀谷[2] 王银磊[1] 杨玛丽[1] 余文贵[3] 颜志明 

机构地区：[1]江苏省农业科学院蔬菜研究所,江苏南京210014 [2]江苏省农业科学院植物保护研究所,江苏南京210014 [3]江苏省农业科学院,江苏南京210014 [4]江苏现代园艺工程技术中心,江苏句容212400

出　　处：《江苏农业学报》2016年第3期631-639,共9页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省农业科技自主创新基金项目[CX(12)1004];基本科研业务专项[ZX(15)2003]

摘　　要：以灰霉菌毒素为选择剂,利用多步筛选法筛选番茄抗性愈伤组织突变体,结果显示,毒素对番茄愈伤组织的诱导、生长具有抑制作用,且随毒素浓度的升高而增强;获得的抗性愈伤组织在不同浓度毒素胁迫下,其增殖率均高于原始型愈伤组织,并在离开选择因子继代培养2代后,仍保持获得的抗性。研究毒素处理后过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)的活性变化发现,无论有无毒素胁迫,抗性愈伤组织突变体及其再生植株的酶活性均高于原始型愈伤组织和原始植株(对照);随着毒素胁迫浓度的增大,抗性愈伤组织突变体及再生植株的3种酶活性有相同的变化趋势,即先升高后降低,且均比对照升高幅度大,其中PAL和PPO酶活性的下降速度较对照慢,说明抗性突变体通过增强与抗病性相关的酶活性,增强植物的防卫能力。Screening of resistant mutants was performed under the stress of Botrytis cinerea toxin by using multistepped positive selections in tomato. The toxin exhibited inhibition to callus induction and growth,and the inhibition was dose-dependent. The growth rate of resistant calli was higher than that of non-resistant calli under various concentrations of toxin,and the resistance was kept stable after the subculture of two generation free of selection factor. The activities of peroxidase（ POD）,polyphenol oxidase（ PPO） and phenylalanine ammonialyase（ PAL） in resistant calli and regenerated plants were higher than those in control regardless of toxin stress.As toxin concentration increased,the activities of 3 enzymes followed an ＂increase-decrease＂trend,and the increment was larger in resistant mutants and regenerated plants,indicating that the resistant mutants improved plant defense by enhancing the activities of disease-risistant enzymes.

关 键 词：番茄 灰霉病 毒素 离体筛选 抗性突变体 防御酶 

分 类 号：S641.2[农业科学—蔬菜学]