基于甲酸和氢氧化钠的衍生移动反应界面的数值计算与实验验证(英文)  

Numerical computation and experimental verification of a derivatized moving reaction boundary originally created with formic buffer and sodium hydroxide

作　　者：吴雪静[1] 金杰[1] 颜炜[1] 肖华[1] 樊柳荫[1] 曹成喜[1] 

机构地区：[1]上海交通大学生命科学技术学院生化分析与生物分离实验室,上海200240

出　　处：《色谱》2016年第8期801-810,共10页Chinese Journal of Chromatography

基　　金：National Natural Science Foundation of China(Nos.21275099,21305087,21475086)

摘　　要：在甲酸(α相)和氢氧化钠(γ相)缓冲液形成的移动反应界面的基础上,提出了一种衍生移动反应界面模型。模型表明在α相和γ相之间会形成一个新的β相,β相和α相形成衍生移动反应界面,β相和γ相形成移动界面。为了验证该模型的有效性,该文给出了相关理论及数值推导过程。此外,基于毛细管电泳和自制装置进行了相关实验。结果表明,若使用以前的移动反应界面,实验结果与理论计算存在较大误差,而采用该文提出的衍生移动反应界面,实验数据与理论计算结果高度一致。该文提出的衍生移动反应界面理论及模型对于电泳,特别是毛细管电泳中样品的分离与富集具有重要的意义。A model of derivatized moving reaction boundary（ MRB）is formulated from original MRB formed with formic buffer（ phase α）and sodium hydroxide（ phase γ）. The model shows the formation of a new phase （ phase β）with high pH value originated from phase γ,derivatized MRB created with phases α and β,as well as stationary boundary formed between phases β and γ. To demonstrate the validity of this model,the theoreti-cal and numerical procedures are advanced. At the same time,the experimental procedures are also developed relied on a capillary electrophoresis（ CE）and a home-made apparatus. There are evident systemic errors and low agreements between the experimental results and theoretical calculation with the original model of MRB de-veloped in our previous papers. However,there are much high agreements between the experiments and theo-retical computation relied on the model of derivatized MRB developed herein. The model of derivatized MRB to-gether with the relative theoretical and experimental procedures holds evident significance for the design of the new separation and compressive techniques of samples used widely in electrophoresis including CE.

关 键 词：电迁移 电泳 数值计算 移动反应界面 堆积 

分 类 号：O658[理学—分析化学]