京海黄鸡中HOXC10基因表达规律及其生物信息学分析  

Tissue Expression and Bioinformatics Analysis of HOXC10 Gene in Jinghai Yellow Chicken

作　　者：张向前[1,2] 董新龙[1,2] 凌姣姣 韩昆鹏[1,2] 张涛[1,2] 王金玉[1,2] 王永娟 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]江苏省动物遗传繁育与分子设计重点实验室,扬州225009 [3]江苏京海集团,南通226103

出　　处：《中国畜牧兽医》2016年第7期1702-1708,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家肉鸡产业技术体系(nycytx-42-G1-05);江苏高校优势学科建设工程;江苏省动物遗传繁育与分子设计重点实验室

摘　　要：同源异型盒基因（homeobox genes,HOX）是在酵母乃至人类等几乎所有的真核细胞中均表达的一类基因。为了探究HOXC10基因表达规律及其生物信息学,采用qRT-PCR方法研究HOXC10基因在8周龄京海黄鸡心脏、肝脏、脾脏、肺脏、肾脏、胸肌、腿肌、下丘脑和卵巢组织中的表达规律,检测其在300日龄高、低产组卵巢组织的表达差异,并通过多种在线软件对原鸡HOXC10基因进行生物信息学分析。结果显示,HOXC10基因在京海黄鸡腿肌中表达丰度极显著高于其他组织（P〈0.01）,且300日龄高产组中HOXC10基因表达丰度显著低于低产组（P〈0.05）。原鸡HOXC10基因序列与火鸡、野鸽、绿头鸭、人、小鼠、牛、猪、山羊及非洲爪蟾的同源性分别为77.4%、94.8%、98.6%、68.3%、67.9%、66.0%、59.0%、68.1%和79.4%;HOXC10基因共编码354个氨基酸,其中丝氨酸的含量最高,潜在的磷酸化位点有24个,在280~342的氨基酸序列内存在一个同源结构域,蛋白质亚细胞主要定位在细胞核中,且不包含跨膜结构。HOXC10基因在京海黄鸡生长和繁殖的基因调控网络中发挥了重要作用。HOX genes express in nearly all eukaryotic cells such as yeast and human.To study tis-sue expression and bioinformatics of HOXC10 gene,the samples of heart,liver,spleen,lung,kid-ney,chest muscle,leg muscle,hypothalamus and ovary tissues were collected from 6 Jinghai Yellow chicken with 8-week-old to analyze the expression pattern of HOXC10 gene in different tissues by qRT-PCR method,and the different expression level were detected in ovary of 300 days old chickens in high yield group and low yield group.The bioinformatics of HOXC10 gene were analyzed by a variety of online softwares of Gallus gallus.The result showed that the expression level of HOXC10 gene in leg muscle was extremely significantly higher than that in other tissues of Jinghai Yellow chicken （P〈0.01）;And it’s expression level in low yield group was signifi-cantly higher than that in high yield group （P〈0.05）;HOXC10 gene of Gallus gallus shared 77.4%,94.8%,98.6%,68.3%,67.9%,66.0%,59.0%,68.1% and 79.4% with Meleagris gal-lopavo,Columba,Anas platyrhynchos,Homo sapiens,Mus musculus,Bos taurus,Sus scrofa, Capraaegagrus hircus and Xenopus laevis,respectively;HOXC10 gene encoded 354 amino acids in which serine was the most abundant;There were 24 potential phosphorylation sites and a HD in 280 to 342 position of amino acid sequence;The subcellular of the encoding protein was mainly located in the cell nucleus,and it had no cross membrane structure.HOXC10 gene might play an important role in regulation network of growth and reproduction in Jinghai Yellow chicken.

关 键 词：HOXC10基因 京海黄鸡 组织表达分析 生物信息学 

分 类 号：Q786[生物学—分子生物学]