奶牛源MRSA菌株的分离鉴定和耐药性分析  

Isolation,Indentification and Antibiotic Resistance Analysis of Methicillin-resistant Staphylococcus aureus(MRSA)from Raw Cow Milk

作　　者：冯清[1] 林伟东[1] 张大帅[1] 戴迪[1] 廖俊[1] 王仙[1] 汪怿旸 梁祥焕 张军 秦爱建[1] 金文杰[1] 

机构地区：[1]扬州大学兽医学院,江苏省动物预防医学重点实验室,江苏省动物重要疫病与人兽共患病防控协同创新中心,扬州225009 [2]扬州大学实验农牧场,扬州225009

出　　处：《中国畜牧兽医》2016年第7期1862-1868,共7页China Animal Husbandry & Veterinary Medicine

基　　金：江苏省“青蓝工程”;扬州市社会发展科技攻关项目(YZ2011091);江苏省农业科技自主创新资金项目(CX(14)2088);江苏高校优势学科建设工程(PAPD);常州市科技支撑项目(CE20152006)

摘　　要：本研究对部分奶牛场采集的502份隐性乳房炎试验(california mastitis test,CMT)检测呈阳性的奶样进行细菌分离、鉴定,运用多重PCR方法筛选出耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA),并对分离出的MRSA用纸片扩散法进行药敏试验,检测其对19种抗菌药物的敏感性。结果显示,分离到细菌的奶样有452份,其中检出葡萄球菌338株,MRSA 89株;MRSA分离株主要对万古霉素、磷霉素、氯霉素和克拉霉素敏感,对青霉素、多黏菌素、大观霉素及头孢他啶具有较强的耐药性。本研究为指导奶牛乳房炎用药、MRSA菌株的快速鉴定及制定MRSA感染的防控措施提供参考。In this study,we collected 502 california mastitis test （CMT）positive milk samples from several cattle farms and then the bacteria were isolated and identified from them.After that, we used multiplex-PCR for screening out methicillin-resistant Staphylococcus aureus （MRSA）. Then we investigated the MRSA’s antibiotics susceptibility to 19 kinds of antibiotics by discdiffu-sion method.The results showed that 338 strains of Staphylococci including 89 strains of MRSA were detected out from the 452 milk samples with bacteria.Additionally,most MRSA showed high sensitivity to vancomycin,fosfomycin,chloramphenicol and clarithromycin,while resistant to penicillin,polymyxin,spectinomycin and ceftazidime.The results provided certain factual basis and method for guiding the use of antibiotics in bovine mastitis,rapid identification of MRSA strains and prevention or control of MRSA infection.

关 键 词：奶牛乳房炎 耐甲氧西林金黄色葡萄球菌 多重PCR 药敏 

分 类 号：S855[农业科学—临床兽医学]