γ-H2AX：一种新型的定量检测CT辐射致DNA损伤的生物标记  

Gamma-H2AX：a novel quantitative biomarker of DNA damage after CT radiation exposure

作　　者：张博[1] 龚建平[1] 张伟[1] 俞泽阳[1] 

机构地区：[1]苏州大学附属第二医院放射科,江苏苏州215004

出　　处：《中华生物医学工程杂志》2016年第1期12-15,共4页Chinese Journal of Biomedical Engineering

基　　金：苏州市科教兴卫青年基金项目（201014）;苏州大学附属第二医院国家自然科学基金预研项目（SDFEYGJ1109）

摘　　要：目的：通过γ-H2AX（H2AX组蛋白异型的磷酸化形式）免疫荧光技术，探讨CT辐射剂量与人外周血单个核细胞γ-H2AX焦点数量之间的相关性。方法64名志愿者肘静脉采集外周血样后，依据CT剂量共设16组（每组4个血样）进行体外CT照射，测定外周血单个核细胞γ-H2AX的表达，并于荧光显微镜下观察计数γ-H2AX焦点。结果体外CT照射后人外周血单个核细胞γ-H2AX焦点数量显著增加，且与CT辐射剂量（DLP）呈线性正相关（R^2＝0.9357，P〈0.05）。结论γ-H2AX能够定量检测CT辐射致外周血DNA损伤，可以作为低剂量电离辐射的生物标志物推广应用。Objective To explore correlation between CT radiation dose and the number of gamma-H2AX （phosphorylated form of H2AX histone variant） foci in human peripheral blood mononuclear cells （PBMC）by usingγ-H2AX-based immunofluorescent assay. Methods 64 volunteers received phlebotomy for peripheral blood samples from cubital vein. Based on CT dose,a total of 16 groups of blood samples were used for in-vitro CT radiation （n=4 samples each）. We measured the expression of PBMC γ-H2AX. Fluorescence microscopy was used for countingγ-H2AX foci. Results After in-vitro CT radiation,there was a significant increase in human PBMCγ-H2AX foci which was in positive linear correlation with the CT dose-length product（DLP）（R^2=0.9357,P〈0.05）. Conclusion γ-H2AX quantitatively reflects CT radiation-induced peripheral blood DNA damage,which is justified for widespread use as a biomarker for low-dose ionizing radiation.

关 键 词：体层摄影术 X线计算机 DNA损伤 

分 类 号：R818[医药卫生—放射医学]