黄芪多糖对种公鸡不同组织miR-16表达的影响及其功能预测分析  

Effects and Bioinformatic Analysis of Astragalus Polysaccharide on miR-16 Expression in Different Tissue of Breeder Cocks

作　　者：任小春[1] 李玉龙[1] 武圣儒 雷新宇[1] 郭伟[1] 党燕娜 龚月生[1] 杨小军[1] 

机构地区：[1]西北农林科技大学动物科技学院,杨凌712100

出　　处：《动物营养学报》2016年第6期1887-1898,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(31272464);教育部新世纪优秀人才项目(NCET-12-0476);陕西省农业公关计划(2014K01-18-02;2015NY149);陕西省科技统筹创新工程计划项目(2015KTCQ02-19)

摘　　要：本试验研究黄芪多糖(APS)对种公鸡不同组织中miR-16表达的影响,结合miR-16预测靶基因的GO和KEGG功能富集性分析,旨在探讨APS对不同组织功能的潜在调控作用。选取1日龄科宝500父母代肉种鸡64只,随机分为对照组和APS组,每组4个重复,每个重复8只鸡。对照组饲喂玉米-豆粕型基础饲粮,APS组在基础饲粮中添加10 g/kg APS,试验期40周。结果表明:1)相比于对照组,APS组可以提高种公鸡的采精量和有效精子数,降低精子畸形率,但差异不显著(P>0.05)。2)miR-16具有组织差异性,APS可显著或极显著上调肝脏、脾脏、十二指肠黏膜、回肠黏膜miR-16的表达(P<0.05或P<0.01),显著降低睾丸miR-16的表达(P<0.05)。3)对miR-16预测靶基因进行GO富集分析,表明预测靶基因在跨膜运输、泛素依赖的蛋白质降解、胞内蛋白运输、糖酵解等物质代谢相关生物过程显著或极显著富集(P<0.05或P<0.01)。KEGG富集分析表明,miR-16靶基因在黏着斑、胰岛素信号通路、糖酵解/糖异生等与机体物质代谢、细胞增殖分化相关通路显著富集(P<0.05),在Toll样受体信号通路、自然杀伤细胞介导的细胞毒性等免疫相关通路也有富集。由此可见,APS上调种公鸡脾脏、肝脏、十二指肠黏膜、回肠黏膜组织的miR-16表达,下调睾丸miR-16表达;miR-16预测靶基因与物质代谢、免疫调控密切相关。APS通过调控种公鸡不同组织miR-16表达,影响机体物质代谢和免疫调控功能。This experiment was conducted to investigate the effects of Astragalus polysaccharides（ APS） on miR-16 expression in different tissues of breeder cocks,combining with the functional enrichment analysis of GO and KEGG pathw ay of miR-16 predicted targets,and to explore the potential regulation role of APS in different tissues via miR-16. A total of 64 one-day-old Cobb 500 breeder cocks were randomly divided into control group and APS group with 4 replicates per group and 8 breeder cocks per replicate. Cocks in the control group were fed a corn-soybean meal based diet,and the others in APS group were fed the basal diet supplemented with 10 g / kg APS. The experiment lasted for 40 weeks. The results showed as follow s： 1） compared with the control group,dietary supplemented with APS improved the semen volume and number of effective sperm,decreased the percentage of abnormal sperm（ P〉0.05）. 2） miR-16 differentially expressed in different tissues; APS ssignificantly increased the miR-16 expression in liver,spleen,duodenum mucosa and ileum mucosa（ P〈0.005 or P〈0.001）,and dow n-regulated miR-16 expression in testis（ P〈0.005）. 3） GO enrichment analysis of miR-16 predicted that targets were showed to be enriched for the metabolic functions in transmembrane transport,ubiquitin-dependent protein catabolic process,intracellular protein transport,glycolytic process（ P〈0.005 or P〈0.001）. KEGG pathway showed that the target genes were enriched in focal adhesion,insulin signaling pathw ay,glycolysis / gluconeogenesis associated with cell proliferation,differentiation and metabolism（ P〈0.005）. And the target genes were also enriched in toll-like receptors signaling pathways and natural killer cell mediated cytotoxicity related to immune pathw ays. In conclusion,APS up-regulates the expression of miR-16 in liver,spleen,duodenum mucosa,ileum mucosa,while dow n-regulates that in testis of breeder cocks.miR-16 predicted targets collect with metabolism and immune regulation,it showes that

关 键 词：黄芪多糖 种公鸡 miR-16 精液品质 组织表达 功能分析 

分 类 号：S831[农业科学—畜牧学]