阿米洛利预处理对浸水束缚大鼠血清NO及胃黏膜MPO的影响  

Effect of amiloride pretreatment on serum NO and gastric mucosal MPO in water immersion restraint rats

作　　者：蒋群[1] 屠伟峰[2] 徐少群[1] 赵高峰[1] 

机构地区：[1]广东省中医院麻醉科,广州510010 [2]广州军区广州总医院临床麻醉中心,广东广州510010

出　　处：《现代医药卫生》2016年第13期1961-1963,共3页Journal of Modern Medicine & Health

基　　金：国家自然科学基金资助项目(81272141);广东省自然科学基金资助项目(S2011010003373);江苏省教育厅开放研究课题(KJS09002)

摘　　要：目的观察酸敏感离子通道阻断剂——阿米洛利(Amiloride)预处理对急性胃黏膜病变(AMGL)大鼠的影响,并探讨AMGL发病机制与酸敏感离子通道(ASICs)的关系。方法选取Wistar大鼠30只,分为正常空白对照组(NC组)、应激模型组(WS组)、阻断剂组(AM组,给予Amiloride预处理),每组10只。采用浸水束缚应激(WIRS)方法复制AMGL模型,于应激6 h后观察胃黏膜大体及光镜下胃黏膜损伤程度、组织学改变及溃疡指数(UI)、血清一氧化碳(NO)活性和胃黏膜组织髓过氧化物酶(MPO)水平。结果 WS组大鼠胃黏膜呈暗红色,有大量点状出血、线状出血或片状糜烂,黏膜下血管和皮区血管充血,光镜下见上皮细胞黏液层变薄、缺失,细胞间隙变大,不同程度坏死;AM组大鼠WIRS 6 h后大鼠胃黏膜出血或糜烂程度明显轻于WS组,光镜下虽可见大量毛细血管充血、扩张,上皮细胞黏膜层有少许缺损,但基本完整。与NC组比较,WS组大鼠WIRS 6 h后胃黏膜UI显著增高,血清NO活性增强,胃组织MPO水平明显增高,差异均有统计学意义(P<0.01);与WS组比较,AM组大鼠胃黏膜UI、血清NO活性及胃组织MPO水平显著降低,差异均有统计学意义(P<0.01)。结论 Amiloride可有效减轻炎症介导的WIRS大鼠急性胃黏膜损伤或病变,提示ASICs可能参与了WIRS大鼠AMGL的发生。Objective To investigate the effect of acid sensing ion channels （ASICs） blocker amiloride precondition on acute gastric mucosal lesion（AGML） rat and to study the relation between AGML pathogenesis with ASICs. Methods Thirty Wistar rats were selected and randomly divided to 3 groups（10 cases per group）,including the normal blank control group（NC）,stress model group（WS） and blocker group（amiloride precondition group,AM）. The AGML model was induced by water immersion re-straint stress（WIRS）. The gastric tissues in general,gastric mucosal lesion degree,histological change under microscope and ulcer index were observed,the levels of serum NO and MPO in gastric mucosal tissue were observed at 6 h after stress. Results The gastric mucosa in the WS group showed dark red with a large of punctate hemorrhage,linear hemorrhage or flake erosion,the sub-mucosal blood vessel and dermatomal blood vessel were congested ,the epithelial cell mucous layer was thin and deleted ,the intra-cellular space was increased with different degrees of necrosis;but the gastric mucosal hemorrhage or erosion degree after 6 h of WIRS in the AM group was obviously improved compared with that in the WS group;a large number of blood capillary congestion and expansion were seen under microscope ,the epithelial cell mucosal layer had a little defects ,but was basically intact. Com-pared with the group NC,the gastric mucosal UI after 6 h of WIRS in the WS group was significantly increased,serum NO en-hanced and gastric tissue MPO level was significantly elevated,the differences were statistically significant（P〈0.01）;compared with the WS group,gastric mucosal UI,serum NO level and gastric tissue MPO level were significantly decreased,the differences were statistically significant（P〈0.01）. Conclusion Amiloride can effectively alleviate the WIRS rat acute gastric mucosal lesion mediated by inflammation,which prompting that ASICs may participate in the occurrence of AGML in WIRS rat.

关 键 词：阿米洛利 胃疾病 急性病 一氧化氮 胃黏膜 过氧化物酶 离子通道 大鼠 

分 类 号：R-332[医药卫生]