诺如病毒67株基因分型  

Genotyping of 67 norovirus strains

作　　者：蒋翠莲[1] 杭惠 艾静[3] 武庆斌[4] 张钧[2] 顾平清[1] 付建光[3] 

机构地区：[1]南京市妇幼保健院检验科,210004 [2]苏州市疾病预防控制中心传防科 [3]江苏省疾病预防控制中心急传所 [4]苏州大学附属儿童医院消化科

出　　处：《中华传染病杂志》2016年第5期280-284,共5页Chinese Journal of Infectious Diseases

基　　金：十二五科教兴卫工程（ZX201109）;江苏医学重点人才基金（RC2011084）

摘　　要：目的了解2014年苏州地区诺如病毒的感染状况及其基因型别。方法收集2014年苏州大学附属儿童医院疑似病毒性腹泻粪便标本322份，反转录（RT ）‐PCR定性检测G Ⅰ和G Ⅱ组诺如病毒，采用聚合酶区（A区）及衣壳蛋白区（C区）引物对诺如病毒阳性标本进行RT‐PCR扩增以鉴定诺如病毒基因型别。通过对相同样本不同区域测序分析得到比较全面的诺如病毒分子特征。结果322份粪便标本中，检测到67株诺如病毒G Ⅱ基因组，未检测到G Ⅰ基因组。A区测序成功42株，G Ⅱ．e型35株，GⅡ．7型3株，G Ⅱ．17型2株，G Ⅱ．12型2株；C区测序成功53株，G Ⅱ．4‐2012Sydney型44株， G Ⅱ．6型4株，G Ⅱ．17型2株，G Ⅱ．3型2株，G Ⅱ．2型1株。结论2014年苏州地区的诺如病毒以G Ⅱ．4‐2012Sydney亚型为主，同时发现G Ⅱ．17型毒株、G Ⅱ．7／G Ⅱ．6和G Ⅱ．12／G Ⅱ．3重组毒株。Objective To investigate the prevalent situations of norovirus infection and genotype distributions in 2014 in Suzhou area .Methods A total of 322 fecal specimens were collected from infants with suspected viral diarrhea at Children′s Hospital of Soochow University in 2014 .Norovirus genogroupⅠ and Ⅱ was detected by reverse transcription （RT ）‐polymerase chain reaction （PCR） ,In an effort to identify norovirus genotypes , RNA dependent RNA polymerase region （region A ） and capsid region （region C） segment of some samples positive for norovirus was amplified by RT‐PCR .Comprehensive molecular characteristics of norovirus were obtained by sequence analysis of the same samples in different regions .Results Among 322 fecal specimens ,67 cases were positive for norovirus of G Ⅱ group ,and norovirus of GⅠ group was not found .The genetic fragments of region A was successfully detected in 42 strains .Among all 42 specimens ,there were 35 GⅡ .e strains ,3 GⅡ .7 strains ,2 GⅡ .17 strains and 2 GⅡ .12 strains .The genetic fragments of region C was successfully detected in 53 strains .Among these 53 specimens ,there were 44 GⅡ .4‐2012Sydney strains ,4 GⅡ .6 strains ,2 GⅡ .17 strains ,2 GⅡ .3 strains and 1 GⅡ .2 strain .Conclusions It′s indicated that G Ⅱ .4‐2012Sydney is the main genotype of norovirus causing viral diarrhea in Suzhou ,and other genotypes including the new GⅡ .17 variant ,GⅡ . 7/GⅡ .6 and GⅡ .12/GⅡ .3 recombinant strains also exist .

关 键 词：诺如病毒 基因分型 GⅡ.4-2012Sydney型 GⅡ.17型Ⅱ 重组 

分 类 号：R725.1[医药卫生—儿科] R440[医药卫生—临床医学]