颅脑损伤合并脑心综合征患者术后感染病原菌分布及对Smad通路蛋白的影响研究  

Pathogen distribution and its effects on serum Smad signaling pathway related proteins in infected cerebrocardiac syndrome patients

作　　者：谈俊[1] 罗超[1] 王旭辉[1] 彭形[1] 王广[1] 吴海波[1] 

机构地区：[1]重庆市中医院脑外科第三军医大学附属大坪医院神经外科,重庆400021

出　　处：《中华医院感染学杂志》2016年第13期2899-2901,共3页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金资助项目(81300965)

摘　　要：目的对颅脑损伤合并脑心综合征患者术后感染病原菌分布及对患者果蝇同源序列蛋白(Smad)信号通路蛋白水平的影响,为临床相关诊断和治疗提供借鉴。方法收集医院2009年10月-2015年4月诊治170例颅脑损伤合并脑心综合征术后感染病原菌患者进行研究并分为感染组,用酶联免疫吸附测定法分析感染后患者血清Smad通路相关蛋白及调节细胞凋亡线粒体蛋白(Smac)和星形胶质源性蛋白(S100B蛋白)水平的变化,以医院同期健康体检者170名为对照组,以未感染颅脑损伤合并脑心综合征患者170例为未感染组。结果170例感染患者共分离病原菌170株,其中革兰阳性菌82株,占48.2%,以F型溶血性链球菌20株占11.8%和化脓性链球菌15株占8.8%为主,革兰阴性菌88株,占51.8%,以阴沟肠杆菌22株占13.0%和肺炎克雷伯菌14株占8.2%为主;感染患者血清Smad通路相关蛋白转化生长因子β(TGFβ)、果蝇同源序列蛋白1(Smad1)、果蝇同源序列蛋白2(Smad2)和果蝇同源序列蛋白3(Smad3)及果蝇同源序列蛋白5(Smad5)表达水平明显高于同期健康体检者和未感染患者而Smac和S100B及β-淀粉样蛋白水平明显降低,且差异有统计学意义(P<0.05)。结论颅脑损伤合并脑心综合征患者术后容易并发病原菌感染,且此过程与Smad通路蛋白及S100B和Smac蛋白表达异常相关。OBJECTIVE To explore the pathogen distribution and its effects on serum Smad signaling transduction related proteins in infected cerebrocardiac syndrome patients,so as to provide reference for clinical diagnosis and treatment.METHODS A total of 170 cases of infected cerebrocardiac syndrome patients in our hospital from Oct.2009 to Apr.2015 were enrolled in this investigation.The isolated pathogens were identified by PHOENIX TM100 equipment and the serum related factors including Smad signaling pathway related proteins,Smac and S100 Bwere assayed by ELISA.Another 170 cases of healthy subjects and 170 cases of non-infected cerebrocardiac syndrome patients were taken as control in our hospital at the same period.RESULTS Totally 170 stains of pathogens were identified from the 170 infected patients.The pathogens contained 82（48.2%）strains of gram-positive bacteria and 88（51.8%）strains of gram-negative bacteria.The gram-positive bacteria mainly were 20 strains of type F hemolytic Streptococcus and 15 strains of Streptococcus pyogenes,accounting for 11.8% and 8.8%.The gram-negative bacteria mainly were 22 strains of Enterobacter cloacae and 14 strains of Klebsiella pneumonia,accounting for 13.0% and 8.2%.The results also revealed that the serum TGFβ,Smad1,Smad2,Smad3 and Smad5were increased greatly in infected patients while the Smac,S100Bandβ-amyloid were decreased dramatically in infected patients（P〈0.05）.CONCLUSION Cerebrocardiac syndrome patients are suffered from pathogen infection,and the abnormal expressions of Smad signaling transduction pathway,S100 Band Smac proteins are involved in this pathological process.

关 键 词：颅脑损伤 脑心综合征 果蝇同源序列蛋白信号通路 β-淀粉样蛋白 

分 类 号：R378[医药卫生—病原生物学]