循环microRNA-21对冠心病不稳定斑块的预测意义  

The relationship between circulating microRNA-21 and unstable plague in patients with coronaryartery disease

作　　者：李韶南[1] 刘震[1] 曾冲[1] 李广镰[1] 罗义[1] 雷晓明[1] 

机构地区：[1]广州市第一人民医院心内科,广州510180

出　　处：《中华急诊医学杂志》2016年第7期878-882,共5页Chinese Journal of Emergency Medicine

基　　金：基金项目：广州市科技计划项目（2012Y2-00025）

摘　　要：目的 探讨冠心病（CAD）患者循环microRNA-21（miRNA-21）相对表达量与冠状动脉内不稳定斑块的关系。方法 连续收集2012年1月至2014年12月在广州市第一人民医院心内科住院并接受冠状动脉造影（CAG）及血管内超声（IVUS）检查的CAD患者100例，根据IVUS检查患者冠脉内是否具有不稳定斑块分为稳定斑块组（SP组）45例、不稳定斑块组（UP组）55例，并选择同期在本院体检中心进行健康体检的50例健康对照病例作为对照组。采用实时荧光定量聚合酶链式反应技术（qRT-PCR）测定入选病例空腹静脉血浆miRNA-21的相对表达量，分析血浆miRNA-21水平与CAD患者不稳定斑块的关系。结果 不稳定斑块组患者血浆miRNA-21相对表达量显著高于稳定斑块组和对照组[（0.87±0.10）vs.（0.78±0.11）vs.（0.67±0.08）, P＜0.05]，将CAD患者血浆miRNA-21水平与患者是否存在不稳定斑块做出受试者工作曲线（ROC）, 血浆miRNA-21的曲线下面积（AUC）为0.869（95%CI： 0.797～0.940）。多因素Logistic回归分析显示血浆miRNA-21是CAD患者存在不稳定斑块的独立预测因子（P＜0.05）。结论 血浆miRNA-21水平升高预示CAD患者冠状动脉内斑块的不稳定状态，是预测CAD患者存在不稳定斑块的重要生物标志物之一。Objective To investigate the relationship between circulating mieroRNA-21 （miR-21） and unstable plague in patients with coronary artery disease （CAD）. Methods A total of 100 consecutive patients with CAD admitted in the department of cardiology in Guangzhou first people＇ s hospital from January 2012 to December 2014 were enrolled. All patients were checked with coronary angiograph and intravascular ultrasound （IVUS） and were divided into two groups according to the presence of unstable plague detected by IVUS： stable plague group （ n = 45 ） and unstable plague group （ n = 55 ）. Another 50 healthy people asking for routine physical examination during the same periods were enrolled as control group. Plasma miRNA-21 of patients were measured by the methods of quantitative reahime fluorescent Polymerase Chain Reaction （qRT-PCR）. Results The levels of plasma miRNA-21 in patients of unstable plague group were significant higher than that in patients of stable plague group and control group （0. 87 ±0. 10） vs. （0. 78 ±0. 11 ） vs. （0. 67 ± 0.08）, P 〈 0. 05. The receiver operation curve （ROC） showed that plasma miRNA- 21 had great significance in assessing the CAD patients with unstable plague evidenced by the areas under the ROC curve to be 0. 869 （95% CI： O. 797 - 0. 940, P 〈 0. 05） . Multivariate logistic regression analysis showed that plasma miRNA-21 was one strong independent predicative factor for unstable plague in patientswith CAD （P〈0.05）. Conclusions The increasing levels of plasma miRNA-21 had the capabilitiy to predicate the unstable coronary plague in CAD patients. That miRNA-21 may be one valuable biomarker for predicating unstable plague in patients with CAD.

关 键 词：微小核糖核苷酸 动脉粥样硬化 冠状动脉疾病 

分 类 号：R541.4[医药卫生—心血管疾病]