榨菜根肿病不同发病程度田根际土壤真菌群落多样性研究  

Study on Rhizosphere Soil Fungal Community in Field With Different Levels of Tuber Mustard Clubroot Disease

作　　者：王殿东[1] 田雪亮[2] 潘丽梅[1] 

机构地区：[1]长江师范学院生命科学与技术学院,重庆408100 [2]河南科技学院资环学院,河南新乡453003

出　　处：《北方园艺》2016年第13期115-118,共4页Northern Horticulture

基　　金：国家自然科学基金资助项目(31170463);重庆市教委青年骨干教师资助计划资助项目

摘　　要：以榨菜根肿病发病程度不同的根际土壤为分离对象,采用454焦磷酸高通量测序方法,研究了榨菜根肿病对根际土壤真菌群落的影响,并对榨菜根际土壤真菌的18SrDNA序列进行大规模测序,揭示根肿病不同程度发病田根际土壤真菌群落的变化特征。结果表明:土壤中子囊菌为优势真菌类群,其次为担子菌;重病田土壤中植物病原真菌数量高于其它发病田;重病田土壤真菌群落的Shannon和Simpson多样性指数、OTU数量和属的数量均低于其它发病田;表明榨菜根肿病在一定程度上影响根际土壤真菌群落的结构,这可能与根肿菌造成榨菜根系破坏后,其根系分泌物旺盛有关。Taking rhizosphere soils of tuber with different levels of tuber mustard clubroot disease as materials,using 454 high throughput sequencing method,the effect of tuber mustard clubroot disease on fungal community in soil and change characteristics were studied.The results showed that the dominant fungal group was Ascomycetes,followed by Basidiomycotain all of the soil samples.The percentage of plant pathogenic fungi in heavy tuber mustard clubroot disease field was higher than other disease fields.The diversity index of Shannon and Simpson and number of OTU in heavy tuber mustard clubroot disease field were lower than others.The result reflected that tuber mustard clubroot could affect rhizosphere soil fungal community to some extent.This was probably related to root exudate of tuber mustard after damaged by Plasmodiophora brassicae.

关 键 词：榨菜根肿病 根际土壤真菌 土传植物病原真菌 多样性指数 

分 类 号：S637.306[农业科学—蔬菜学]