盐酸纳美芬对肺缺血-再灌注损伤大鼠肺组织β-内啡肽与IL-8表达的影响  

Effect of nalmefene hydrochloride on the expressions of pulmonary β - endorphin and IL - 8 in rats with lung ischemia - reperfnsion injury

作　　者：杨敏[1] 徐标[1] 曾昆[1] 

机构地区：[1]华中科技大学同济医学院附属普爱医院(西区),湖北武汉430035

出　　处：《中国急救医学》2016年第6期561-565,I0003,共6页Chinese Journal of Critical Care Medicine

摘　　要：目的研究盐酸纳美芬抑制肺缺血-再灌注损伤的作用及其机制。方法将50只大鼠随机均分为模型组、高剂量纳美芬组、低剂量纳美芬组、地塞米松组、假手术组共五组，每组10只。模型组采用阻断左肺门法成功建立肺缺血-再灌注模型。高、低剂量纳美芬组、地塞米松组大鼠分别于模型建立时尾静脉注射纳美芬（20μg／kg、10μg／kg）及地塞米松（5mg／kg）。假手术组大鼠不阻断肺门，不予任何治疗。各组大鼠于再灌注6h后检测其动脉血气值并处死大鼠，留取左肺上叶组织观察各组大鼠肺组织损伤程度，检测其肺组织湿／干质量比值、MDA、SOD、β-内啡肽及IL-8表达。结果与模型组比较，高、低剂量纳美芬组、地塞米松组动脉血PCO2值、肺组织损伤程度、湿／干质量比值、MDA、β-内啡肽、IL-8表达均显著降低（P〈0．05或P〈0．01），动脉血PO2值、SOD表达显著升高（P〈0．05或P〈0．01）。与地塞米松组比较，低剂量纳美芬组动脉血PCO2值、肺组织损伤程度、湿／干质量比值、MDA、β-内啡肽表达均显著降低（P〈0．05），IL-8表达无明显变化（P〉0．05），动脉血PO2值、SOD表达显著升高（P〈0．05）。与低剂量纳美芬组比较，高剂量组动脉血PCO2值、肺组织损伤程度、湿／干重比值、MDA、β-内啡肽、IL-8表达均显著降低（P〈0．05），动脉血PO2值、SOD表达显著升高（P〈0．05）。结论盐酸纳美芬可抑制肺缺血-再灌注损伤，其作用可呈剂量依赖性，其机制之-可能为减少肺组织内β-内啡肽生成、减轻氧化应激及炎症反应程度。Objective To study the inhibition of nalmefene hydrochloride on lung ischemia - reperfusion injury and its mechanism. Methods Fifty rats were randomly divided into model group, high dose of nalmefene group, low dose nalmefene group, dexamethasone group and sham operation group equally（ n = 10 ）. The lung ischemia - reperfusion model was established by occlusion of the left pulmonary hilum in the model group. The intravenous injection of nalmefene （20 μg,/kg, 10 μg/kg） and dexamethasone （5 mg/kg） was applied when the model was established in the high dose of nalmefene group, the low dose of nalmefene group and the dexamethasone group respectively. The sham operation group without occlusion of the left pulmonary hilum was not given any treatment. At 6 h after reperfusion, all rats were detected arterial blood gas value and then sacrificed. The specimens from the upper lobe of the left lung tissue were preserved to observe pulmonary lesions, detect the ratio of wet / dry weight and the expressions of MDA, SOD, β - endorphin and IL - 8 in lung tissue. ResultsCompared with the model group, the value of PCO2, the degree of pulmonary lesions, the ratio of wet / dry weight and the expressions of MDA, β - endorphin and IL - 8 in lung tissue were significantly decreased （P 〈 0.05 or P 〈 0.01 ）, but the value of PO2 and the expression of SOD was significantly increased （P 〈 0.05 or P 〈 0.01 ） in the high or low dose nalmefene group and the dexamethasone group. Compared with dexamethasone group, the value of PCO2 , the degree of pulmonary lesions, the ratio of wet / dry weight and the expressions of MDA, β - endorphin and IL - 8 in lung tissue were significantly decreased （P 〈 0.05 ）, the value of PO2 and the expression of SOD was significantly increased （P 〈 0.05 ） , but the expression of IL - 8 was not significantly changed in the low dose nalmefene group（P 〉 0.05）. Compared with the low dose of nalmefene group, the value of PCO2, the degree of pulmonary lesions, the ratio

关 键 词：盐酸纳美芬 肺缺血-再灌注损伤 Β-内啡肽 氧化应激 白细胞介素-8 

分 类 号：R655.3[医药卫生—外科学]