养心通脉方含药血清对家兔骨髓内皮祖细胞增殖、迁移和黏附能力的影响  

Impact of Serum Containing Yangxin Tongmai Decoction(养心通脉方) on Multiplication,Migration and Adhesion Ability of Rabbits' Bone Marrow Endothelial Progenitor Cells

作　　者：卢健棋[1] 林露南 李苏依[1] 温志浩[1] 杨清华[1] 何新兵[1] 韩景波[1] 张振千[1] 刘靖[3] 

机构地区：[1]广西中医药大学第一附属医院 [2]玉林市中医院 [3]广西中医药大学

出　　处：《中医杂志》2016年第14期1242-1246,共5页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81260522);广西自然科学基金(2013GXNSFAA019193)

摘　　要：目的探讨养心通脉方治疗冠心病的可能作用机制。方法 4只家兔随机分为含药血清组[养心通脉方8ml/（kg·d）]和空白血清组（等体积生理盐水）,每组连续灌胃5天,末次给药0.5 h后颈总动脉取血,制备养心通脉方含药血清和空白血清。观察不同剂量（1.25%、2.5%、5%、10%）和干预时间（12 h、24 h、48 h、72 h、96 h）的含药血清对家兔骨髓内皮祖细胞（EPCs）线粒体活性的影响,确定养心通脉方含药血清最佳干预剂量和时间。EPCs调整细胞密度至5×10~5个/ml,分为治疗组和对照组,两组分别用最佳剂量的养心通脉方含药血清和空白血清培养,干预最佳时间后,观察两组EPCs增殖、迁移、黏附能力。结果与对照组同浓度比较,治疗组2.5%、5%、10%浓度含药血清均能明显增强EPCs线粒体活性,且治疗组随浓度升高EPCs线粒体活性越高（P〈0.05）,故10%浓度为最佳干预浓度。与对照组同时间点比较,治疗组在48 h、72 h、96 h时EPCs线粒体活性均明显升高,治疗组与本组前一时间点比较,72 h较48 h及96 h较72 h时EPCs线粒体活性明显升高（P〈0.05）,故最佳干预时间为96 h。两组分别用10%养心通脉方含药血清和10%空白血清培养96 h,治疗组EPCs的增殖、迁移、黏附能力均明显高于对照组（P〈0.05）。结论养心通脉方含药血清能明显提升EPCs的增殖、迁移、黏附能力,进而促进缺血心肌血管新生,这可能是其治疗冠心病的作用机制之一。Objective To explore the possible mechanism of Yangxin Tongmai Decoction（养心通脉方） in the treatment of coronary heart disease. Methods Four rabbits were randomized into the serum containing medicine[8 ml /（ kg·d） of Yangxin Tongmai Decoction]group and the blank serum group（ equivoluminal normal saline）.Both groups were given intragastric administration for 5 days. After 0. 5 hour of the last administration,blood in common carotid artery was sampled to prepare serum containing Yangxin Tongmai Decoction and blank serum. Impact of serum containing medicine with different doses（ 1. 25%,2. 5%,5%,or 10%） and different intervening time（ 12 h,24 h,48 h,72 h,or 96 h） on the mitochondrial activity of rabbits’ bone marrow endothelial progenitor cells（ EPCs） was examined. The optimal intervening dose and time of serum containing Yangxin Tongmai Decoction was tested. After the cell density was regulated to 5 × 105 cells / ml,EPCs were divided into the treatment group and the control group. The two groups were cultured with the optimal dose of serum containing Yangxin Tongmai Decoction and blank serum respectively. After inventing for the optimal time,the multiplication,migration and adhesion ability of EPCs in both groups were examined. Results Compared with the control group at the same concentration,serum containing medicine at the concentration of 2. 5%,5% and 10% could enhance the mitochondrial activity of EPCs.With the increasing concentration,the mitochondrial activity was enhanced in the treatment group（ P 〈 0. 05）. Thus10% was the best intervening concentration. Compared with the control group at the same time point,the mitochondrial activity of EPCs was enhanced in the treatment group at 48 h,72 h and 96 h. Compared the treatment group at previous time points,the mitochondria of EPCs was more active at 72 h than that at 48 h,and more active at 96 h than that at 72 h（ P 〈 0. 05）. Thus the best intervening time was 96 h. The two groups were cultured with serum containing10

关 键 词：冠心病 内皮祖细胞 养心通脉方 线粒体活力 细胞增殖 细胞迁移 细胞黏附 

分 类 号：R259[医药卫生—中西医结合]