雪上一枝蒿甲素微透析探针体外回收率研究  

In vitro recovery rate of bullatine A microdialysis probe

作　　者：唐红艳[1] 陈晓兰[1] 吴玉梅[1] 魏文珍 戴雅洁 

机构地区：[1]贵阳中医学院药学院,贵州贵阳550002

出　　处：《中国中药杂志》2016年第13期2538-2542,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81260646);贵州省科学技术基金项目(黔科合J字[2012]2066号);贵州省中医药管理局中医药;民族医药科学技术研究课题(QZYY2011-58);贵阳中医学院博士基金项目

摘　　要：该研究建立UPLC-MS/MS测定雪上一枝蒿甲素微透析探针回收率的分析方法,采用浓度差法(增量法,减量法)测定其体外回收率,并考察灌流液的p H,流速,浓度,温度对回收率的影响,以探讨微透析技术用于雪上一枝蒿甲素体内药动学研究的可行性。该试验建立的UPLC-MS/MS分析方法在要求范围内线性良好,色谱的专属性、回收率、精密度等均符合微透析样品的研究要求。增量法和减量法测得回收率无显著差异;相同条件下,探针的体外回收率随着流速的增加而降低,随着温度的升高而明显增加,与探针周围雪上一枝蒿甲素的浓度无关。结果表明,微透析技术可用于雪上一枝蒿甲素药动学研究,反透析法即减量法可用于雪上一枝蒿甲素体内微透析回收率的测定。To establish UPLC-MS/MS method for determination of the recovery rate of bullatine A microdialysis probe. The concentration difference method（ incremental method,decrement method） was used to measure in vitro recoveries,and the effects of perfusate p H value,flow rate,concentration,and temperature on the recovery rate were investigated to explore the feasibility of microdialysis for the pharmacokinetic study of bullatine A. The method of UPLC-MS / MS showed good linear relationship within the required range; the specificity,recovery rate and precision of chromatography met the requirements of microdialysis samples. There was no significant difference in the measured recovery rate between incremental method and decrement method. Under the same conditions,in vitro recovery rate of the probe was decreased with the increase of flow rate,and was significantly increased with the increase of temperature,but was independent of bullatine A concentrations around the probe. The results showed that,microdialysis technology can be used for the pharmacokinetic study of bullatine A,and retrodialysis method（ decrement method） can be used for the determination of the in vivo recovery rate of bullatine A microdialysis.

关 键 词：雪上一枝蒿甲素 微透析 回收率 

分 类 号：R285[医药卫生—中药学]