抗菌剂马波沙星对家蚕细菌病的防治效果  

Control Effect of Antibacterial Agent Marbofloxacin on Silkworm Bacterial Diseases

作　　者：刘文光[1] 衣葵花[1] 刘惠芬[1] 于振诚[1] 李智峰[1] 郭光[1] 李云芝[1] 

机构地区：[1]山东省蚕业研究所,山东烟台264002

出　　处：《蚕业科学》2016年第3期466-472,共7页ACTA SERICOLOGICA SINICA

基　　金：山东省现代农业产业技术体系蚕桑产业创新团队建设项目;山东省重点研发计划项目(No.2015GNC111001)

摘　　要：马波沙星是一种新型的氟喹诺酮类抗菌剂,对革兰阴性菌、革兰阳性菌和支原体均有抑制或杀灭作用。通过体外抑菌试验和生物试验探究马波沙星对家蚕主要细菌病害的防治效果。马波沙星对苏芸金芽孢杆菌(Bacillus thuringiensis)、黑胸败血芽孢杆菌(Bacillus bombysepticus)、沙雷铁氏菌(俗称灵菌,Serratia marcescens)的体外最低抑菌浓度(MIC)分别为0.25 mg/L、0.50 mg/L、0.25 mg/L,最低杀菌浓度(MBC)分别为8 mg/L、16 mg/L、32 mg/L,均大于或等于盐酸环丙沙星的MIC和MBC值。以质量浓度为50 mg/L、200 mg/L的马波沙星药液给5龄起蚕添食8 h,对由苏芸金芽孢杆菌、黑胸败血芽孢杆菌感染引起的家蚕败血病的防治效率为100%;以800 mg/L马波沙星药液给5龄起蚕添食24 h或连续3 d每日给药8 h,对由灵菌感染引起的家蚕败血病的防治效率为100%,防治效果显著优于盐酸环丙沙星(P<0.05)。5龄起蚕接种苏芸金芽孢杆菌3 h后添食50 mg/L马波沙星药液24 h,对家蚕败血病的防治效率为100%;5龄起蚕接种黑胸败血芽孢杆菌1 h后添食100 mg/L马波沙星药液24 h和接种3 h后添食200 mg/L马波沙星药液24 h,对家蚕败血病的防治效率均可达90.0%以上;5龄起蚕接种灵菌3 h后添食1 600 mg/L马波沙星药液24 h,对家蚕败血病的防治效率可达90.0%以上,防治效果显著优于盐酸环丙沙星(P<0.05)。药物对家蚕的毒性试验表明,给3龄、4龄、5龄家蚕幼虫连续添食200 mg/L、600 mg/L、1 000 mg/L的马波沙星药液,对家蚕的生长发育和茧质无不良影响。试验结果初步显示,马波沙星对家蚕无安全风险,可以用于家蚕细菌性败血病的防治。Marbofloxacin is a novel fluoroquinolone antibacterial agent,which has inhibitory action and killing effect on gram-negative bacteria,gram-positive bacteria and mycoplasma. In this study,the control efficacy of marbofloxacin on main bacterial diseases of the silkworm（ Bombyx mori） was investigated by in vitro bacteriostatic test and biological test.The results showed that the minimal inhibitory concentration（ MIC） of marbofloxacin against Bacillus thuringiensis,Bacillus bombysepticus and Serratia marcescens were 0. 25 mg / L,0. 50 mg / L,and 0. 25 mg / L,respectively,and the minimal bacteriocidal concentration（ MBC） were 8 mg / L,16 mg / L,and 32 mg / L,respectively,which were higher than orequal to that of ciprofloxacin hydrochloride. At 8 h after the newly exuviated larvae of the 5th instar were fed with marbofloxacin of 50 mg / L and 200 mg / L, the control effect on silkworm septicemia caused by Bacillus thuringiensis and Bacillus bombysepticus was 100%. The control effect on silkworm septicemia caused by Serratia marcescens was 100% through continuous administration of 800 mg / L marbofloxacin to the newly exuviated larvaeof the 5th instar for 24 h or for 8 h per day in 3 days,being significantly higher than that of ciprofloxacin hydrochloride（ P〈 0. 05）. The control effect on silkworm septicemia was 100% by administration of 50 mg / L marbofloxacin for 24 h to the newly exuviated larvae of the 5th instar at 3 h after inoculating Bacillus thuringiensis. The control effect on silkworm septicemia was all over 90. 0% by administration of 100 mg / L marbofloxacin for 24 h to the newly exuviated larvae of the 5th instar at 1 h after inoculating Bacillus bombysepticus and by administration of 200 mg / L marbofloxacin for 24 h to the newly exuviated larvae of the 5th silkworm instar at 3 h after inoculating Bacillus bombysepticus. The control effect on silkworm septicemia was over 90. 0% by administration of 1 600 mg / L marbofloxacin for 24 h to the newly exuviated larvae of the 5th instar at 3 h

关 键 词：抗菌药物 马波沙星 家蚕细菌性败血病 防治效果 安全性 

分 类 号：S884.4[农业科学—特种经济动物饲养]