CCR9／CCL25途径在小鼠皮肤移植急性排斥反应中的表达及意义  

The expression and significance of CCR9/CCL25 pathway in acute rejection of mouse skin transplantation

作　　者：李杰[1] 李海平[1] 李转转[1] 靳玉洁[1] 张吉才[1] 

机构地区：[1]十堰市太和医院,湖北十堰442000

出　　处：《中华器官移植杂志》2016年第2期106-111,共6页Chinese Journal of Organ Transplantation

基　　金：湖北省教育厅中青年人才项目（Q20152102）

摘　　要：目的探讨CCR9／CCL25途径在小鼠皮肤移植术后急性排斥反应时的表达及意义。方法以BALB／C小鼠和C57BL／6小鼠分别作为同种异体皮肤移植和同系皮肤移植的供鼠，以C57BL／6小鼠为受者，建立小鼠皮肤移植模型，术后给予部分同种异体移植受鼠尾静脉注射抗CCL25中和抗体（实验组）或IgG同型对照抗体（对照组），剂量为2μg／只。每2天注射1次，共注射10次。观察各组受鼠移植皮片的存活时间，并在移植后3、5、7d收集各组受鼠的移植皮片和脾脏，通过HE染色检测移植皮片的炎症反应程度；采用免疫组织化学法及共聚焦检测确定CCR9表达水平与表达部位。结果同种异体移植受鼠移植皮片中存在广泛的炎症细胞浸润；免疫组织化学及共聚焦检测显示同种异体移植组受鼠移植皮片及脾脏高表达CCR9蛋白，且主要表达于CD3＋T淋巴细胞表面，给予受鼠尾静脉注射抗CCL25中和抗体可明显延长同种异体移植受鼠移植皮片的存活时间，并降低移植皮片的炎症反应程度。结论同种异体皮肤移植术后CCR9高表达于移植皮片及脾脏T淋巴细胞中，给予抗CCL25抗体可明显延长移植皮片的存活时间，CCR9／CCL25途径在小鼠皮肤移植急性排斥反应中具有重要作用。Objective To investigate the expression and significance of CCR9/CCL25 pathway in acute rejection of mouse skin transplantation. Method BALB/c mice and C57BL/6 mice were selected as allogeneic and syngeneic skin graft donors and C57BL/6 as recipients, then established a murine skin transplantation model of .acute rejection. Allogeneic transplant recipient mice were injected with an Anti- CCL25（2 g, as experimental group） mAb or IgG（2 g, as control group） every other day via tail vein, a total of 10 injections. The transplanted graft was scored visually daily, and then we collected skin graft and spleen from recipient mice of each group at 3,5,7 days after transplantation. HE staining was done to analyze necrosis of skin tissue Confocal and immunohistochemistry were also used to measure CCR9 and CCL25 expression in recipient skin grafts Result HE staining indicated that there was a widespread inflammatory cell infiltration in the skin from allo-transplantation mice, and CCR9 expression measured by immunohistochemistry and confocal was significantly elevated in the surface of the infiltrated CD3 ＋ T cells from skin grafts tissue and spleen. Neutralization of CCL25 with Anti-CCL25 mAb significantly prolonged allogra,ft survival and markedly reduced inflammation. Conclusion CCR9 was highly expressed in the spleen and skin grafts tissue of allogeneic transplanted mice Neutralization of CCL25 by intravenous injection of Anti-CCL25 monoclonal antibody significantly prolonged skin allograft survival. Our study indicates that CCR9/CCL25 pathway is involved in acute rejection process of skin transplantation model in mice were used as syngeneic and skin graft skin graft donor mice.

关 键 词：小鼠 皮肤移植 移植物排斥 趋化因子 CCR9 

分 类 号：R622.1[医药卫生—整形外科]