胶体钯在原子吸收光谱法测定全血中铅、镉、锰应用研究  

Study on the application of colloidal palladium in the determination of lead,cadmium and manganese in blood by atomic absorption spectrometry

作　　者：董明[1] 张爱华[1] 孙毅[1] 杨展鸿 潘巧裕[1] 罗晓婷[1] 

机构地区：[1]广东省职业病防治院,广东省职业病防治重点实验室,广东广州510300

出　　处：《中国职业医学》2016年第3期341-344,349,共5页China Occupational Medicine

基　　金：国家科技支撑计划(2014BAI12B01);国家临床重点专科建设项目(2011-09);广东省化学中毒与核辐射突发事件医学救援应急技术研究中心(2012A032100002);广东省职业病防治重点实验室(2012A061400007);广东省医学科研基金(B2014018);广东省医学科研基金指令性项目(C2014011)

摘　　要：目的研究以胶体钯作为基体改进剂在石墨炉原子吸收光谱法测定全血中铅、镉、锰的应用效果。方法用血样稀释剂将全血样品稀释10倍后,以胶体钯为基体改进剂,优化待测元素的灰化和原子化温度后,以石墨炉原子吸收光谱仪检测全血中铅、镉、锰水平,并与普通钯盐作为基体改进剂的应用效果进行比较。结果胶体钯作为基体改进剂的最佳用量为5.00μL;其可将全血中铅、镉、锰的灰化温度分别提高至900、800和1 400℃,将原子化温度分别提高至1 900、1 800和2 000℃;与普通钯盐比较,胶体钯使3种待测元素有更宽的灰化和原子化温度范围。本方法全血中铅、镉、锰分别在质量浓度0.12～100.00、0.05～4.00和0.02～10.00μg/L呈良好线性关系,相关系数均大于0.999;最低检出浓度分别为1.20、0.50和0.20μg/L（以样品稀释10倍计）;平均加标回收率分别为99.2%～104.0%、102.8%～105.5%和98.3%～103.2%;批内相对标准偏差（RSD）分别为1.2%～2.6%、2.7%～5.3%和2.3%～2.8%,批间RSD分别为1.6%～3.9%、4.0%～6.1%和3.2%～4.4%。结论胶体钯用于测定全血中的铅、镉、锰,背景干扰小,有利于改善检测的精密度和灵敏度,是一种优良的基体改进剂。Objective To study the application of colloidal palladium as a matrix modifier in the determination of lead,cadmium and manganese in whole blood by graphite furnace atomic absorption spectrometry（ GFAAS）. Methods The whole blood samples were diluted 10 times by blood diluents,and then the colloidal palladium was applied as a chemical modifier to improve the ashing and atomizing temperature. The levels of lead,cadmium and manganese in whole blood were determined by GFAAS. The effects of palladium chloride as a conventional matrix modifier were compared. Results The optimal quantity of colloidal palladium as a matrix modifier was 5. 00 μL. It can raise the ashing temperatures of lead,cadmium and manganese to 900,800 and 1 400 ℃,respectively; and raised their atomizing temperatures to 1 900,1 800,and 2 000 ℃,respectively. Compared to the palladium chloride,the colloidal palladium can provide wider ashing temperature and atomizing temperature of the above 3 elements. The good linearity ranges of lead,cadmium and manganese were 0. 12-100. 00,0. 05-4. 00 and 0. 02-10. 00 μg / L,respectively. All the correlation coefficients were greater than0. 999. The minimum detectable concentrations were 1. 20,0. 50 and 0. 20 μg / L,respectively（ samples were diluted 10times）. The recovery rates were 99. 2%-104. 0%,102. 8%-105. 5% and 98. 3%-103. 2%,respectively. The relative standard deviations（ RSDs） of within-run precision were 1. 2%-2. 6%,2. 7%-5. 3% and 2. 3%-2. 8%,respectively,and the RSDs of between-run precision were 1. 6%-3. 9%,4. 0%-6. 1% and 3. 2%-4. 4%,respectively. Conclusion Colloidal palladium was an excellent matrix modifier in determining the levels of lead,cadmium and manganese in whole blood. It has low background interference and can improve the accuracy and precision of detection.

关 键 词：胶体钯 全血 铅 镉 锰 基体改进剂 

分 类 号：R136.3[医药卫生—劳动卫生]