铜绿假单胞菌催化阿糖2,6-二氨基嘌呤核苷合成阿糖鸟苷  

Synthesis of Guanine Arabinoside from 2,6-Diaminopurine Arabinoside by Pseudomonas aeruginosa TX16

作　　者：刘国生[1] 许航[1] 曹倩[1] 邢善涛 黎梦[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]新乡拓新生化股份有限公司,河南新乡453000

出　　处：《中国医药工业杂志》2016年第7期857-860,共4页Chinese Journal of Pharmaceuticals

基　　金：河南省高校科技创新团队支持计划(13IRTSTHN009);河南省教育厅自然科学基金项目(2011B180032)

摘　　要：利用铜绿假单胞菌(Pseudomonas aeruginosa TX16)细胞催化阿糖2,6-二氨基嘌呤核苷进行脱氨反应转化生成阿糖鸟苷。经优化得到的最适酶促反应条件为:在160 mmol/L磷酸盐缓冲液(p H 7.5)中加入13%铜绿假单胞菌TX16细胞和30 mmol/L阿糖2,6-二氨基嘌呤核苷,反应温度60℃,反应120 h。在上述条件下,嘌呤核苷的转化率可达90.0%。Guanine arabinoside were synthesized from 2,6-diaminopurine arabinoside with Pseudomonas aeruginosa TX16 ceils as catalytic agent. Through optimization experiments, the optimal reaction conditions were obtained as follows： adding 13% Pseudomonas aeruginosa TX16 bacterial cells and 30 mmol/L 2,6-diaminopurine arabinoside into the 160 mmol/L phosphate buffer solution （pH 7.5）, at the reaction temperature of 60℃ and reacting for 120 h. Under the above conditions, the conversion rate ofpurine nucleoside was 90.0%.

关 键 词：阿糖2 6-二氨基嘌呤核苷 阿糖鸟苷 铜绿假单胞菌 生物合成 

分 类 号：Q814.2[生物学—生物工程]