氯化甲基汞对成年大鼠脑组织中GFAP和MBP的影响  

Effect of different doses of methyl-mercuric chloride on GFAP,MBP of adult rats in different courses

作　　者：范玉芹 吕伟[2] 王树才[2] 曹秉振[2] 胡怀强[2] 

机构地区：[1]山东省妇幼保健院,山东济南250014 [2]中国人民解放军济南军区总医院,山东济南250031

出　　处：《工业卫生与职业病》2016年第4期270-272,共3页Industrial Health and Occupational Diseases

基　　金：中国博士后科学基金资助(项目编号:20080431409)

摘　　要：目的探讨不同剂量氯化甲基汞蓄积对成年大鼠脑组织胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、髓鞘碱性蛋白(myelin basic protein,MBP)的影响。方法 120只SD大鼠随机分为正常对照组、氯化甲基汞小剂量组(0.5mg/kg)、氯化甲基汞中剂量组(1.0mg/kg)、氯化甲基汞大剂量组(2.0mg/kg)。每天灌胃1次,连续染毒,各组染毒时间分别为1、2、4周。采用免疫组织化学方法观察不同时间点各组大鼠脑组织中GFAP、MBP的变化。结果各染毒时间组中氯化甲基汞剂量增加,大鼠脑组织GFAP表达升高和MBP表达降低,差异均有统计学意义(P<0.01)。同一剂量组不同染毒时间比较,随染毒时间延长,GFAP表达升高;MBP表达降低,差异均有统计学意义(P<0.01)。结论氯化甲基汞促进星形胶质细胞增殖,破坏髓鞘,并与汞的蓄积量与染毒时间相关。Objective To investigate the effect of different doses of methyl-mercuric chloride（MMC）on glial fibrillary acidic protein（GFAP）and myelin basic protein（MBP）of adult rats in different courses.Methods 120 Sprague Dawley rats were randomly divided into the normal control group and MMC low dose group（0.5mg/kg）,middle dose（1mg/Kg）,high dose（2mg/kg）.MMC group rats were given by different dose MMC gavage once a day for 1w,2w and 4w respectively.GFAP and MBP were measured by immunohistochemistry at different times after administrating various dosages.Results The GFAP expression increased and MBP expression decreased with MMC dosage in various times,the differences of groups were significant（P〈0.01）.As the time went on,the GFAP expression increased and MBP expression decreased in the same dose group,the differences of groups were significant（P〈0.01）.Conclusions MMC can destroy myelin sheath and promote GFAP,which shows a significant dose-effect and time-effect correlation.

关 键 词：氯化甲基汞 胶质纤维酸性蛋白 髓鞘碱性蛋白 汞中毒 

分 类 号：R135.13[医药卫生—劳动卫生]