荒漠肉苁蓉和管花肉苁蓉指纹图谱比较研究  

Fingerprint Comparative Analysis of Cistanche deserticola Y.C. Ma and Cistanche tubulosa(Schrenk) Wight

作　　者：朱乃亮[1] 徐荣[1] 吴海峰[1] 马国需[1] 朱寅荻[1] 彭芳[1] 王夏[1] 任小敏[1] 陈君[1] 许旭东[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室,北京100193

出　　处：《中国药学杂志》2016年第13期1116-1119,共4页Chinese Pharmaceutical Journal

基　　金："重大新药创制"科技重大专项"十二五"计划(综合性新药研究开发技术大平台)资助项目(2012ZX09301-002-001-026):中国医学科学院北京协和医学院药用植物研究所创新团队发展计划资助

摘　　要：目的建立荒漠肉苁蓉和管花肉苁蓉药材的超高效液相色谱法(UPLC)指纹图谱,并进行比较研究。方法采用ACQUITY UPLC~ BEH C_(18)色谱柱(2.1 mm×50 mm,1.7μm),乙腈-0.2%甲酸水梯度洗脱,流速:0.4 m L·min^(-1),检测波长为240 nm,柱温:40℃,采用对照品指认进行成分分析。结果建立了两种肉苁蓉药材的超高效液相色谱法(UPLC)指纹图谱,标定了15个共有峰,鉴定其中5个共有峰,10批荒漠肉苁蓉药材相似度在0.667到0.905之间,10批管花肉苁蓉药材相似度在0.249到0.991之间,药典收载的两种肉苁蓉指纹图谱具有显著区别。结论本法简便,重现性好,可用于区分荒漠肉苁蓉和管花肉苁蓉药材及药材的质量评价。OBJECTIVE To establish the UPLC fingerprint of Cistanche deserticola Y.C. Ma and Cistanche tubulosa （Schrenk） Wight. METHODS The UPLC fingerprints of 10 batches of C. deserticola and C. tubulosa were determined on an ACQUITY UPLC BEH C18 column （2. 1 mm x50 ram,1.7 μm） eluted with the mobile phase consisting of 0. 2% formic acid solution and acetonitrile in gradient mode at a flow rate of 0. 4 mL·min-1, the column temperature was 40℃, and the detection wavelength was set at 240 nm. The chemical attribution of the fingerprint was determined by reference substance comparison method. RESULTS The common mode of the UPLC fingerprint of C. deserticola and C. tubulosa was set up under the established conditiom. There were 15 common peaks in the fingerprints of 10 samples, five of which were identified. The similarities varied from 0. 667 to 0. 905 and from 0. 249 to 0. 991 for Cistanche deserticola Y. C. Ma and Cistanche tubulosa （Schrenk） Wight, respectively. There was significant difference in the fingerprints between C. deserticola and C. tubulosa. CONCLUSION The method is simple and reliable, which can be readily utilized for distinguishing C. deserticola from C. tubulosa and their quality control.

关 键 词：肉苁蓉 指纹图谱 超高效液相色谱法 质量评价 

分 类 号：R284[医药卫生—中药学]