原子吸收光谱测定全血铅的应用  

In whole blood testing by atomic absorption spectrometry

作　　者：陈美珠[1] 谢恺[1] 郭萍[1] 张声灿[1] 张琦 

机构地区：[1]龙岩市疾病预防控制中心检验科,福建龙岩364000

出　　处：《中国城乡企业卫生》2016年第6期50-52,共3页Chinese Journal of Urban and Rural Enterprise Hygiene

摘　　要：目的改进《血铅临床检验技术规范》（简称规范）推荐的GFAAS测定血铅的方法,提高灵敏度和准确度,使该法应用更为可行、可靠。方法用接近空白的基底血制备标准系列,用1%硝酸和含铵盐与曲拉通的化学基体改进剂（简称基改）稀释血样,与石墨炉原子吸收光谱测定血铅含量。结果血铅浓度0~80μg/L内,线性良好,回归方程y=0.007 14x＋0.016 6,相关系数r为0.999 4,方法检出限1.7μg/L,定量检出限5.7μg/L,加标回收率97.6%~100.9%,精密度2.48%~5.69%。结论本法线性范围及相关系数、检出限、精密度、准确度、室内质控,参加省主管单位盲样考核合格,均符合《规范》的要求,适用于日常工作的开展。Objective To imporve the method of determination of blood lead by using GFAAS, which is recommended by ＂the technical specification for blood lead clinical examination＂, to improve the sensitivity, accuracy and feasibility of the method. Methods Used the basal blood of preparing standard series which is close to blank, 1% HNO3 and containing ammonium salt and Triton chemical matrix modifier（GM） diluted blood were given, by graphite furnace atomic absorption spectra to determine lead content in blood. Results Blood lead concentration of 0 ~ 80 μg / L and good linearity, the regression equation y = 0.007 14x＋0.016 6, the correlation coefficient is 0.999 4, and the detection limit of 1.7 μg/L and quantitative detection limit of 5.7 μg/L, and the recovery rate of 97.6%~100.9%, precision 2.48%~5.69%Conclusion The modified method in linear range and the correlation coefficient, detection limit, precision,accuracy, quality control, participation in the provincial competent unit blind test are all qualified, and in line with the ＂standard＂ requirements, it＇s available to use in practice.

关 键 词：血铅 GFAAS 基改 

分 类 号：R135[医药卫生—劳动卫生]