响应面优化香椿子多酚清除亚硝酸盐及动力学初步研究  

Study on the Nitrites Scavenging Conditions of Polyphenols from Seeds of Toona Sinensis by Response Surface Method and its Preliminary Kinetics

作　　者：赵磊[1] 程燕妮[1] 姜喆[1] 李万忠[1] 

机构地区：[1]潍坊医学院药学院,山东潍坊261053

出　　处：《中医药导报》2016年第14期47-50,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(81274049)

摘　　要：目的:研究模拟胃环境下香椿子多酚清除亚硝酸盐条件及初步动力学。方法:在单因素实验基础上选取不同因素与水平,根据中心组合实验设计,采用响应面分析法,确定影响清除率因子;对香椿子多酚与茶多酚清除动力学曲线进行数学模型拟合。结果:所得响应面优化清除条件为胃蛋白酶浓度3.75 mg/m L,反应时间107.03 min,多酚浓度0.58 mg/m L,此时清除率为93.23%;香椿子多酚与茶多酚清除动力学曲线符合Logistic模型及零级动力学方程。结论:香椿子多酚在模拟胃环境下对亚硝酸盐具有较高清除作用,为下一步开发应用香椿子多酚提供理论基础。Objective： To study on the Nitrites scavenging conditions of polyphenols from seeds of toona sinensis by Response Surface Method and its Preliminary kinetics under simulating acid environment of stomach. Methods： Based on the single factor experiment, the different factors and levels were selected, and response surface analysis method was used to determine the impact factor on the scavenging by Box-Benhnken design. Mathematical models were studied fitting for removal kinetics curve of polyphenols from seeds of toona sinensis and tea. Results： Design expert analysis indicated that the optimal processing conditions were： pepsin concentration of 2.05 mg/mL, reaction time of 11.903 min, polyphenols concentration of 0.55 mg/mL, and the scavenging rate was 96.23%. The scavenging kinetic curve of polyphenols from seeds of toona sinensis and tea conforms logistic model and zero order kinetics equation. Polyphenols from seeds of toona sinensis have a higher scavenging effect on nitrite under simulating acid environment of stomach. Conclusions： The study can be provided theoretical basis for the development and application of polyphenols from seeds of toona sinensis.

关 键 词：香椿子多酚 亚硝酸盐 响应面分析 清除动力学 

分 类 号：R284[医药卫生—中药学]