氯化锂联合hUC-MSCs移植对阿尔茨海默病小鼠的治疗作用  

Effect of lithium chloride combined with hUC-MSCs transplantation on Alzheimer's disease mouse

作　　者：孟楠[1] 马珊珊[2] 王欣欣[1] 姚宁[2] 邢衢 宋及时 黄团结 关方霞[1,2] 

机构地区：[1]郑州大学第一附属医院干细胞研究室,郑州450052 [2]郑州大学生命科学学院干细胞研究室,郑州450001

出　　处：《郑州大学学报（医学版）》2016年第2期157-161,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目81471306;U1404313;河南省高校科技创新团队项目15IRTSTHN022;河南省科技创新人才计划项目154200510008

摘　　要：目的:研究氯化锂(LiCl)联合人脐带间充质干细胞(hUC-MSCs)移植对阿尔茨海默病小鼠(APP^+转基因小鼠)的干预作用。方法:PCR鉴定为APP^+转基因小鼠40只随机分为4组:APP^+组、hUC-MSCs移植组、LiCl注射组、联合处理组(LiCl注射^+hUC-MSCs移植)。处理1个月后,Morris水迷宫检测小鼠的认知水平,酶标仪法和分光光度法检测小鼠血清中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,qRT-PCR和Western blot法检测小鼠海马组织中Wnt/β-catenin通路相关因子的表达。结果:与APP^+组比较,LiCl注射组APP^+小鼠逃避潜伏期缩短,穿越平台次数增多,平台所在象限停留时间延长,MDA含量下降,小鼠海马组织中β-catenin、c-Myc mRNA和蛋白表达量增加,GSK-3βmRNA和蛋白表达量降低(P均<0.05);hUC-MSCs移植可使APP^+小鼠血清中SOD活性升高(P均<0.05),其他指标的变化与LiCl注射组相同;LiCl注射联合hUC-MSCs移植对平台所在象限停留时间、MDA含量、c-Myc蛋白表达的影响具有协同效应(P<0.05)。结论:LiCl联合hUC-MSCs移植对APP^+转基因小鼠具有明显的治疗作用。Aim：To research the effects of lithium chloride （ LiCl） combined with human umbilical cord mesenchymal stem cells（hUC-MSCs） transplantation on Alzheimer＇s disease model mice（APP^＋transgenic mice） and the potential mech-anism.Methods：A total of 40 APP^＋mice identified by PCR were allocated into 4 groups randomly：APP^＋group, hUC-MSCs group, LiCl group and LiCl combined with hUC-MSCs group.After 1 month, all mice were to study the learning and memory ability by Morris water maze test , the activity of superoxide dismutase （ SOD） and the concentration of malonalde-hyde（MDA） in the serum were determined by microplate spectrophotometer and ultraviolet spectrophotometer , the mRNA and proteins associated with Wnt/β-catenin pathway were detected by qRT-PCR and Western blot .Results： Compared with APP^＋group, the escaping latency was significantly shorter , the times of crossing platform was significantly increased and the time spent in the platform quadrant was significantly longer ,MDA concentration was decreased ,the expressions of β-catenin and c-Myc mRNA and proteins in the hippocampus tissue were increased , while GSK-3βmRNA and proteins ex-pression was decreased in LiCl group;in hUC-MSCs group ,the changing trend of the indexes mentioned above were similar with the LiCl group , and the activity of SOD in serum increased;hUC-MSCs transplantation and LiCl had synergistic effects on the time spent in the platform quadrant, MDA concentration and c-Myc protein expression（P〈0.05）.Conclusion：LiCl combined with hUC-MSCs transplantation has therapeutic effects on APP ＋transgenic mice .

关 键 词：氯化锂 WNT/Β-CATENIN通路 干细胞移植 阿尔茨海默病 小鼠 

分 类 号：R592[医药卫生—老年医学]