瑞舒伐他汀对心肌肥厚大鼠心肌组织Beclin-1蛋白表达的影响  

Effect of rosuvastatin on expression of Beclin-1 in myocardial tissue of rats with myocardial hypertrophy

作　　者：丁同斌[1] 简立国[1] 张丽华[1] 刘士超[1] 赵江涛[1] 程栋[1] 

机构地区：[1]郑州大学第二附属医院心内科,郑州450014

出　　处：《郑州大学学报（医学版）》2016年第2期251-254,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:探讨瑞舒伐他汀对心肌肥厚大鼠心肌组织中自噬相关蛋白Beclin-1表达的影响。方法:健康雄性SD大鼠75只,随机分为假手术组、模型组和RSV组,每组25只,模型组和RSV组通过腹主动脉缩窄术建立心肌肥厚模型,术后分别给予生理盐水和瑞舒伐他汀(4 mg/kg)灌胃,1次/d,连用4周。4周后测定心脏指数,用RT-PCR和Western blot法分别检测大鼠左心室心肌组织中Beclin-1 mRNA和蛋白的表达水平。结果:与假手术组比较,模型组大鼠心肌明显肥厚,心脏指数升高,心肌组织中Beclin-1 mRNA和蛋白表达上调(P<0.05);与模型组比较,RSV组大鼠心肌无明显肥厚,心脏指数降低,心肌组织中Beclin-1 mRNA和蛋白表达下降(P<0.05)。结论:瑞舒伐他汀可抑制压力超负荷性心肌肥厚,其作用机制可能与降低自噬水平有关。Aim：To investigate the effect of rosuvastatin （ RSV） treatment on Beclin-1 expression in myocardial tissue＆amp;nbsp;of rats with myocardial hypertrophy due to autophagy .Methods：A total of 75 healthy male SD rats were randomly allocated into 3 groups：sham operation group , model group , and RSV group , 25 rats in each group .Model group and RSV group were given normal saline and RSV （4 mg/kg） by gavage,respectively, one time a day and for 4 weeks.The heart samples were collected after four weeks to detect the expressions of Beclin-1 mRNA and protein using RT-PCR and Western blot . Results：Compared with sham operation group , myocardial hypertrophy , cardiac index and the expressions of Beclin-1 mR-NA and protein in the model group increased （P〈0.05）.Compared with the model group, there was no obvious cardiac hypertrophy,and cardiac index,the expressions of Beclin-1 mRNA and protein were lower in RSV group（P〈0.05）.Con-clusion：RSV could inhibit pressure overload cardiac hypertrophy , and the mechanism may be related with the decreased expression of Beclin-1.

关 键 词：瑞舒伐他汀 压力超负荷 心肌肥厚 BECLIN-1 大鼠 自噬 

分 类 号：R542.2[医药卫生—心血管疾病]