陈皮、半夏对衰老脐静脉内皮细胞中PI3K-Akt和磷酸化p38表达的影响  

Effect of Citrus and Pinellia on the expression of PI3K-Akt and p-p38 in aging human umbilical vein endothelial cells

作　　者：黄小波[1] 王宁群[1] 陈玉静[1] 赵荣博[1] 陈文强[1] 

机构地区：[1]首都医科大学宣武医院中医科,北京100053

出　　处：《北京中医药》2016年第5期411-414,共4页Beijing Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81574037);北京市自然科学基金项目(7162079);北京市卫生系统高层次卫生技术人才培养计划项目(2013-2-31)

摘　　要：目的研究陈皮、半夏对衰老脐静脉内皮细胞(HUVEC)中磷脂酰肌醇3激酶(PI3K)-蛋白激酶B(Akt)信号通路和磷酸化p38表达的影响。方法 0.25%胰岛素-柠檬酸盐诱导建立HUVEC衰老模型。采用Western blot方法检测陈皮、半夏干预后衰老HUVEC中PI3K、磷酸化Akt(p-Akt)和磷酸化p38(p-p38)的表达水平。结果与空白对照组和陈皮半夏对照组比较,诱导衰老组PI3K、p-Akt和p-p38表达显著升高(P<0.01);陈皮半夏干预组、PI3K阻滞组PI3K、p-Akt和p-p38表达均较诱导衰老组显著降低(P<0.05,P<0.01)。结论陈皮、半夏对衰老HUVEC中PI3K-Akt通路和磷酸化p38的表达有抑制作用。Objective To study the influence of Citrus and Pinellia on signaling pathway of Phosphatidylinositol-3 kinase （PI3K） - protein kinase B（Akt） and the expression of p-p38 in aging human umbilical vein endothelial cells（HUVEC）. Methods 0. 25% insulin-citrate was used to induce the aging model of HUVEC. Then the expressions of PI3K, p-Akt, and p-p38 in aging HUVEC after intervention by Citrus and Pinellia were observed by Western blot. Results Compared with the control group and Citrus and Pinellia control group, the expressions of PI3K, p-Akt and p-p38 were significantly increased in the model group （P 〈 0. 01 ）. Compared with the model group, the expressions of PI3K, p-Akt and p-p38 were significantly decreased in Citrus and Pinellia group and PI3K block group （P 〈0. 05, P 〈0.01 ）. Conclusion Citrus and Pinellia could inhibit the PI3K-Akt pathway and the expressions of p-p38 in aging HUVEC.

关 键 词：陈皮 半夏 衰老 脐静脉内皮细胞 磷脂酰肌醇3激酶 蛋白激酶B p38 

分 类 号：R285[医药卫生—中药学]