裸小鼠肝癌原位移植模型的建立  

Establishment of an orthotopic implantation model of hepatocellular carcinoma in nude mice

作　　者：田树红[1] 王日超 邢桂兰[1] 肖敏[1] 符健[1] 

机构地区：[1]海南医学院海南省药物安全性评价研究中心,海口571199

出　　处：《中国实验动物学报》2016年第3期239-242,247,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金资助项目(NO 81160408)

摘　　要：目的建立一种BALB/c裸小鼠肝癌原位移植模型。方法采用异氟烷气化对实验动物进行全身麻醉,分别用"包埋法"和"夹心法"建立BALB/c裸小鼠肝癌原位移植瘤模型。手术后用小动物活体成像系统对肿瘤组织的生长情况进行检测,4周后对肝及肿瘤组织进行组织病理学检查。结果采用夹心法建立的肝癌原位移植BALB/c裸小鼠模型,具有操作时间短,难度低,成瘤率高的特点,肿瘤组织生长良好,在腹腔内未出现肿瘤广泛种植现象,组织病理学检查无异常,动物死亡率低等特点。结论与包埋法相比,夹心法更具优势,可快速制备大批量小鼠肝癌原位移植模型,为肝癌原位药效学及其他研究提供更便利的平台。Objective To establish a new orthotopic implantation model of hepatocellular carcinoma in BALB/c nude mice. Methods The nude mice were under general anesthesia with isoflurane inhalation. Hepatocellular carcinoma was induced by subcutaneous inoculation of human hepatocellular carcinoma Bel-7402 cells in nude mice. The orthotopic implantation model of hepatocellular carcinoma in nude mice was established by orthotopic implantation of small pieces of the liver cancer tissues into the mouse liver by either a sandwich method or embedding method. The post-operative tumor growth was determined by a small animal in vivo imaging system. Histopathological examination of the liver and tumor tissues was performed at four weeks after modelling. Results The orthotopic hepatocellular carcinoma model of nude mice established by the sandwich method had several distinct advantages： shorter operative time,less operational difficulties,with a high rate of tumor formation,no wide-spread peritoneal tumor dissemination,well-preserved tumor histological structure,and low animal mortality. Conclusions Compared with the embedding method,the sandwich method can save much time for preparation of this orthotopic implantation model of hepatocellular carcinoma in nude mice,and provide a more convenient platform for research of liver cancer pharmacodynamics and other biological studies.

关 键 词：BALB/C裸鼠 肝癌 原位移植 小动物活体成像系统 

分 类 号：Q95-33[生物学—动物学]