口服补液盐降低氯化锂-匹罗卡品惊厥持续状态大鼠死亡率的研究  

Mortality of rat models of LiCl-pilocarpine-induced status epilepticus can be reduced by oral rehydration salt solution

作　　者：韩慰[1] 蒋莉[1] 何蓉[1] 宋晓洁[1] 程莉[1] 

机构地区：[1]重庆医科大学附属儿童医院神经内科,儿童发育疾病研究教育部重点实验室,儿童发育重大疾病国家国际科技合作基地,儿科学重庆市重点实验室,重庆400014

出　　处：《中国实验动物学报》2016年第3期243-247,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(NO.81371452);重庆市科委自然科学基金重点项目(cstc2013jj B0031);教育部博士点基金课题(20125503110011)

摘　　要：目的探讨通过胃管灌胃改良口服补液盐（oral rehydration salts,ORS）降低氯化锂-匹罗卡品诱发惊厥持续状态（status epilepticus,SE）模型的死亡率的方法。方法选用成年组（60 d）SD大鼠30只和幼年组（20 d）SD大鼠45只,随机分为成年SE＋ORS组、成年SE组、幼年SE＋ORS组、幼年SE组、幼年血糖组各15只。经腹腔注射氯化锂和匹罗卡品,诱发大鼠惊厥发作,惊厥60 min后,给予安定止惊。各ORS组于SE后1、12、24 h根据不同年龄予以不同剂量改良ORS灌胃。其余各组不给予处理。记录实验大鼠惊厥发作情况,惊厥前后体重变化及死亡情况;血糖测定组于SE后8 h取血检测血糖,探索降低该模型死亡率的方法。结果 （1）各组惊厥后体重均降低,约占体重的5%~8%。（2）氯化锂-匹罗卡品惊厥持续状态模型死亡率较高,幼年组40.00%,成年组57.14%;死亡发生时间集中在惊厥后24 h内,72 h后死亡数趋于平稳。（3）SE后实验大鼠血糖明显降低,给予ORS液灌胃后,血糖趋于正常水平。（4）以改良ORS灌胃后,幼年组和成年组72 h内死亡率明显降低。结论 SE后改良ORS液灌胃有效改善实验动物死亡率,是有效提高其生存率的方法。Objective To explore a new method of reducing the mortality of rat models of LiCl-pilocarpine-induced status epilepticus（ SE） by oral gavage of rehydration salts（ ORS） liquid. Methods Forty-five young SD rats（ 20-dayold） and 30 adult SD rats（ 60-day-old） were enrolled in this study. They were randomly divided into five groups： the adult SE rats received ORS gavage（ adult SE ＋ ORS）,adult SE rats（ adult SE） and young SE rats received ORS gavage（ young SE ＋ ORS）,young SE rats（ young SE） and young blood glucose group. Seizures were induced in both young and adult SE groups with i. p. LiCl-pilocarpine was injected i. p. to induce SE,which was interrupted by diazepam at 60 minutes after SE onset. ORS gavage was conducted in all the ORS groups in different doses according to their ages at 1 hour,12 hours or 24 hours after the onset of SE. The other groups were not given any treatment. The latency of seizure onset,changes in body weight,level of blood glucose at 8 hours after SE onset and finally the death rate of the rats were recorded. Results（ 1）The body weight of the rats with seizures was reduced by 5%- 8%.（ 2） The mortality rate of the SE groups was high,especially at 24 hours after the SE onset,which was 40% in the young group and 57% in the adult group.（ 3） The blood glucose level was significantly decreased in the SE rats,and ORS gavage made the blood glucose returning to normal in the SE rats.（ 4） ORS gavage apparently decreased the mortality rate of young and adult groups during the 72 hours after seizure onset. Conclusions ORS gavage effectively reduces the mortality of SE rats,and is an effective method to improve the survival of SE rats.

关 键 词：氯化锂-匹罗卡品 惊厥持续状态模型 大鼠 死亡率 

分 类 号：Q95-33[生物学—动物学]