雄性裸鼹鼠可育个体与不可育个体生殖系统结构与功能差异初探  

Structural and functional differences of the reproductive system in fertile and infertile male naked mole rats

作　　者：余琛琳[1] 林丽芳[1] 袁子彦[1] 赵善民[1] 汤球[1] 孙伟[1] 蔡丽萍[1] 徐晨[1] 崔淑芳[1] 

机构地区：[1]第二军医大学实验动物中心,上海200433

出　　处：《中国实验动物学报》2016年第3期248-252,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家科技支撑计划(No.2015BAI09B02);2014年度军队实验动物专项科研课题[No.SYDW〔2014〕005号);No.SYDW(2014)007号]联合资助

摘　　要：目的观察和分析雄性裸鼹鼠中可育个体和不可育个体生殖系统结构和功能的差异,初步探索雄性不可育个体不能繁育后代的原因。方法雄性裸鼹鼠可育个体和不可育个体各5只,先采集一侧睾丸和附睾组织,称重后换算脏器系数;再采集另一侧睾丸组织,用中性甲醛溶液进行固定后制备组织切片;取另一侧附睾用作精子计数、精子活动度的测定;采集血清测定血清中黄体生成素(LH)和睾酮(T)浓度。结果与可育个体组相比较,不可育个体组的睾丸重量下降(P<0.05),附睾重量显著下降(P<0.01),且睾丸系数和附睾系数均显著下降(P<0.01);精子数量显著减少(P<0.01),精子活动度也显著减弱(P<0.01);血清黄体生成素和睾酮水平均显著降低(P<0.01);睾丸组织切片观察显示,不可育个体各级生精细胞严重脱落,排列混乱无序,支持细胞和间质细胞均有减少。结论雄性裸鼹鼠不可育个体生殖系统存在睾丸和附睾萎缩、生精功能下降和性激素分泌减少等现象。Objective The aim of this study was to observe and analyze the differences of reproductive system in fertile and infertile male naked mole rats. Methods Five fertile and 5 infertile 2-5-year old male naked mole rats were used in this study. The testis and epididymis on one side were collected,weighted,and measured to get their viscera coefficient. The other side testicle was collected and fixed in neutral formalin solution for histological examination. The other side epididymis was used to examine the sperm count and sperm activity. Serum of the naked mole rats was collected to measure the level of luteinizing hormone（ LH） and testosterone（ T）. Results Compared with the fertile group,the testis weight of infertile group was significantly decreased（ P〈0. 05）,their epididymis weight,testicular coefficient and the epididymis coefficient were all significantly decreased（ P〈0. 01）,all their sperm count and sperm activity and their LH and T levels were significantly decreased（ P〈0. 01 for all）. Pathological examination of the testicular tissues of the fertile group showed that many types of spermatogenic cells were seriously detached,the numbers of Sertoli cells and stromal cells were decreased. Mature sperms in the seminiferous tubules were highly decreased in number or even completely disappeared.Conclusions Compared with the fertile individuals,the reproductive system of infertile male naked mole rats presents structural atrophy and declined function.

关 键 词：裸鼹鼠 可育个体 不可育个体 雄性生殖系统 精子发生 黄体生成素 睾酮 

分 类 号：Q95-33[生物学—动物学]