抗纤灵方抑制肾络病慢性肾衰竭大鼠TGF-β/P13K/Akt信号旁路的实验研究  

Experimental Research on “Kang Xian Ling Decoction” to Inhibit TGF-β/P13K/Akt Signal Passage of Rats with Chronic Renal Failure

作　　者：王瑞鑫[1] 陈刚[1] 何立群[1] 

机构地区：[1]上海中医药大学附属曙光医院肾病科,上海200021

出　　处：《中国中西医结合肾病杂志》2016年第6期480-483,I0001,共5页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：上海市教育委员会科研创新项目(No.12YZ069);上海市科学技术委员会科研计划项目(No.11ZR1437700);国家自然科学基金资助项目(No.81173615);国家临床重点专科;国家中医药管理局重点学科;上海市进一步加快中医药事业发展三年行动计划(2014年~2016年)建设项目(No.ZY3-LCPT-1-1006)

摘　　要：目的:采用5/6肾切除方法建立慢性肾衰竭大鼠模型,观察抗纤灵对5/6肾切除大鼠肾组织中TGF-β/PI3k/Akt信号通路的影响。方法:将SD大鼠分为正常组、模型组(生理盐水)、治疗组(抗纤灵)各12只,除正常组外均采用5/6肾切除方法建立慢性肾衰竭大鼠模型,疗程2个月。应用生化、放射免疫法、western blot等技术,检测肾功能、24 h尿蛋白定量、肾脏病理,检测TGF-β/P13K/Akt信号通路蛋白表达。结果:实验结束时,与模型组相比较,抗纤灵组的血肌酐数值显著下降(P<0.01)、蛋白尿下降差异有统计学意义(P<0.05);病理MASSON染色:抗纤灵组肾小球肥大减弱,毛细血管基底膜增厚减轻,系膜基质明显减少,胶原纤维成分低于模型组;western blot检测,与模型组相比,抗纤灵组的p-Akt灰度比值显著下降。结论:抗纤灵方可能通过抑制TGF-β/PI3K/Akt信号通路,从而有效地降低5/6肾切除大鼠的血肌酐,减轻肾组织纤维化、改善肾功能、降低蛋白尿。Objective： Replicate 5 /6 nephrectomy CRF rat＇s models to observe the effects on TGF- β / P13 K / Akt signal passage of experimental rats with ＂Kang Xian Ling Decoction＂. Methods： Divide 36 rats into three groups（ normal group,model group with physiological saline,treatment group with the drug）; then replicate 5 /6 nephrectomy CRF rat＇s models except normal group for 2 months; apply medical technology such as biochemical mensuration of blood and organization,Radioimmunoassay and Western blot to detect renal function and 24 h UPro,wrenal pathology and TGF- β / P13 K / Akt signal passage. Results： After experiment,compared with model group,serum creatinineresults of mode group dramatically reduced（ P 0. 01）,serum creatinine of treatment group increase obviously（ P 0. 05）,Pathology observation MASSON; compared with model group,Hypertrophy on glomerulus and basilar membrane on capillary vessel of treatment group has weakened. There has been an obvious decrease in mesentery stroma;collagenous fibre elements is lower than model group; During western blot examination,compared with model group,gray value of p-Akt in treatment group decline obviously. Conclusion： Chinese medicine ＂Kang Xian Ling Decoction＂can significantly improve kidney function of 5 /6 nephrectomy CRF rats through reducing albuminuria and serum creatinine to protect renal function with inhibiting TGF- β / P13 K / Akt signal passage.

关 键 词：抗纤灵方 慢性肾衰竭 PI3K/AKT信号通路 肾纤维化 

分 类 号：R285.5[医药卫生—中药学]