低强度振动载荷刺激对于三维多孔钛合金内成骨细胞粘附和增殖的影响  

Effect of Low-level Mechanical Vibration on Proliferation and Attachment of Osteoblasts in 3-D Porous Titanium Implants

作　　者：高贺奇 曹璐[1] 唐振宏 翟明明[1] 刘娟[1] 罗二平[1] 景达[1] 

机构地区：[1]第四军医大学生物医学工程系,陕西西安710032 [2]陕西省医疗器械检测中心,陕西西安710061

出　　处：《现代生物医学进展》2016年第22期4233-4236,共4页Progress in Modern Biomedicine

基　　金：陕西省自然科学基金项目(2014JQ4139);国家自然科学基金项目(31270889)

摘　　要：目的：探究低强度振动载荷刺激对于三维多孔钛合金（porous titanium,p T i）内兔成骨细胞（osteoblasts,O B）粘附和增殖的影响,以期为临床振动载荷协同p T i治疗骨缺损提供重要的实验依据。方法：原代分离培养1日龄新生新西兰兔颅盖骨O B,待传至第3-6代以5×1 04/m L密度植入p T i中,随机分为振动组和对照组。振动组在5%C O2、37℃温度环境下进行3天低强度振动载荷（0.5 g,30 H z）刺激,每天1 h;对照组放置在无载荷的振动平台上,分别采用D A PI核染色法和M T T法评价O B在p T i中的粘附和增殖情况。结果：在振动载荷刺激下,每个视野下O B细胞在p T i上的粘附数量1 4±3个,对照组粘附数量6±2个,粘附能力显著增强（P〈0.05）;在振动载荷刺激下,p T i上的O B细胞增殖吸光度为36.5%±0.8%,对照组吸光度为34%±1%,细胞增殖能力得到了显著促进（P〈0.05）。结论：低强度振动载荷刺激对于提高p T i中的O B细胞生物学活性具有促进作用,本研究为下一步系统探索振动载荷协同p T i对于骨缺损的修复效果奠定了基础。Objective： To determine the effect of low-level mechanical vibration on proliferation and attachment of osteoblasts in the 3-D porous titanium implants （pTi）, and thus provide critical experimental basis for combinative application of mechanical vibration and pTi in the treatment of osseous defects in clinics. Methods： Primary osteoblasts（OB） were obtained by digesting the calvarial bone of 1-day-old New Zealand rabbits. Cells with passages 3-6 were used in the experiment. Primary osteoblasts were seeded onto the pTi implants at a density of 5x 104 cells/mL, and randomLy divided into the control and mechanical vibration group. Cells in the mechanical vibration group were subjected to 1 h/day mechanical vibratory stimulation （0.5 g, 30 Hz） for 3 days at room temperature. Cells in the control group were placed onto the inactivated mechanical loading platform. MTT assays were performed to determine the cell proliferation and DAPI assays were performed to evaluate the cell attachment in pTi. Results： The DAPI staining results demonstrate that the osteoblastic attachment number in pTi in the mechanical vibration group was significantly higher than the control group （P〈0.05）. MTT assays show that the mechanical vibration group showed increased cell proliferation as compared with the control group. Conclusions： Our present study shows that mechanical vibration improved the biological activities of osteoblasts in pTi, which lays the foundation of the systemic investigation of pTi combined with mechanical vibration in bone defect repair.

关 键 词：低强度振动载荷 多孔钛合金 成骨细胞 细胞增殖 

分 类 号：R68[医药卫生—骨科学] R318.08[医药卫生—外科学]