羟基磷灰石Zeta电位对其蛋白吸附性能的影响  

Effect of Zeta Potential of Hydroxyapatite on Protein Adsorption

作　　者：付亚康 李锡忠[1] 夏晓[1] 樊先茂 刘耀文[3] 翁杰[2] 

机构地区：[1]雅安职业技术学院,四川雅安625000 [2]西南交通大学材料先进技术教育部重点实验室,四川成都610031 [3]四川农业大学食品学院,四川雅安625014

出　　处：《现代生物医学进展》2016年第19期3610-3613,共4页Progress in Modern Biomedicine

基　　金：国家重点基础研究发展计划(2012CB933600);国家自然科学基金项目(51172188);四川省高校科研创新团队建设计划项目(14TD0050)

摘　　要：目的:为了寻找羟基磷灰石(HA)颗粒的蛋白吸附性能与其Zeta电位之间的关系,为研发具有优秀骨修复能力的HA材料提供理论依据。方法:本文采用水热反应法,合成了形状规则的带状HA颗粒,并采用SEM、BET、XRD对其进行表征。检测了HA颗粒在不同p H、PO_4^(3-)浓度中的Zeta电位;选择小牛血清白蛋白(BSA)、溶菌酶(LYS)两种蛋白作为模型蛋白,测试了HA颗粒在不同p H、PO_4^(3-)浓度环境中对BSA或LYS的吸附量。结果:随着吸附体系的p H值逐渐增大,HA颗粒表面负电性迅速增加,HA颗粒对BSA的吸附能力逐渐减弱,对LYS的吸附能力逐渐增强。随着吸附体系中PO_4^(3-)浓度逐渐增大,HA颗粒表面负电性呈先增大后减小的变化趋势,对BSA、LYS的吸附性能均逐渐减弱。结论:p H和PO_4^(3-)浓度的变化,都能引起HA颗粒Zeta电位的变化,前者引起静电作用类型、强度的变化而改变HA蛋白吸附性能;后者引入竞争吸附,蛋白吸附量随竞争离子浓度增多而逐渐减少。Objective： In order to research the correlation between surface voltage and protein adsorption property of hydroxyapatite（HA）, provide valuable evidences for developing HA materials with excellent ability of bone repair. Methods： HA particles were hydrothermally synthesized and characterized by SEM、BET and XRD. The Zeta potentials of HA in different p H or concentration of PO_4^（3-）were examined. The protein adsorption amounts of negatively charged bovine serum albumin（BSA） and positively charged lysozyme（LYS） on HA in different p H or concentration of PO_4^（3-）were examined. Results： The results indicated that as the increasing of p H in the adsorption system, the negative charge on HA surface also increased quickly and BSA adsorption ability slowly weakened, but the LYS adsorption ability gradually intensified. It was also found out that as the increasing of PO_4^（3-）concentration in the adsorption system, the negative charge on HA surface showed a trend of first increasing and then decreasing, and the protein adsorption abilities of BSA and LYS both weakened gradually. Conclusion： The changes of p H and PO_4^（3-）concentration can both regulate the Zeta potential of HA particles, the former is caused by the changes of the type and intensity of the electrostatic interaction, which regulate the protein adsorption capability of HA; the latter is due to the introducing of competition adsorption, and the protein adsorption amount decreases with the increasing of the competition ion concentration.

关 键 词：羟基磷灰石 ZETA电位 蛋白吸附 静电作用 竞争吸附 

分 类 号：R318[医药卫生—生物医学工程] TQ174[医药卫生—基础医学]