365nm长波紫外光辐照对人嗜中性粒细胞内钙离子浓度的影响  

The Effect of 365 nm Long-wave Ultraviolet Light Irradiation on Intracellular Calcium Concentration in Human Neutrophils

作　　者：李存波[1] 陈民才[2] 史学琛 魏巍[1] 苏兴磊 李哲明[1] 潘雷霆[1,3] 

机构地区：[1]南开大学物理科学学院泰达应用物理研究院,天津300457 [2]中国人民解放军307医院输血科,北京100071 [3]深圳市生物医学光学微纳检测与成像重点实验室光电工程学院深圳大学,广东深圳518060

出　　处：《南开大学学报（自然科学版）》2016年第3期53-58,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：格尔木市科技局社会发展基金;国家自然科学基金(11574165)

摘　　要：紫外光在临床医学上的众多应用中最值得关注的是紫外光照射自血回输疗法,但其具体机制尚未清楚.基于倒置荧光显微镜光路,在单细胞水平上,分别以连续式和间隔式365 nm长波紫外光辐照射血液中数量最多的白细胞-嗜中性粒细胞,检测其对细胞内钙离子浓度的影响.结果表明,相对低剂量的长波紫外光辐照会使胞内钙浓度升高,而较高剂量的紫外光可导致胞内钙浓度下降,甚至低于起始钙浓度.此外,胞外无钙的情况下,紫外仍可引起胞内钙升高,说明紫外引起胞内钙库释放导致胞内钙浓度升高.为探讨紫外光照射自血回输疗法的作用机理提供一定的实验支持.As a physical therapy, ultraviolet blood irradiation（UBI） is generally regarded as one of the most successful application in clinics. However, the underlying mechanisms remain largely unclear. In the present work, we investigated the effect of continuous and discontinuous 365 nm ultraviolet A（UVA） on cytosolic calcium concentration（[Ca^2＋]c） in human neutrophils at the single-cell level based on the inverted fluorescence microscope. The results showed that low-dose UVA irradiation induced [Ca^2 ＋]c increase, in contrast, high-dose UVA irradiation led to [Ca^2 ＋]c decrease. In addition, external calcium had no obvious effect on UVA-induced [Ca^2 ＋]c increase, indicating that calcium store release make a greater contribution to this [Ca^2＋]c increase. This work on regulation [Ca^2＋]c of neutrophils by UVA might be helpful in the research of UBI.

关 键 词：长波紫外光 嗜中性粒细胞 钙信号 紫外光照射自血回输疗法 

分 类 号：Q63[生物学—生物物理学]