不同林龄陈山红心杉土壤微生物群落结构特征  

Soil microbial community structure characteristics of Chenshan Red-heart Chinese fir of different stand ages

作　　者：刘顺[1,2] 吴珍花 郭晓敏[1] 盛可银 朱新传 温卫华[1] 张文元[1] 

机构地区：[1]江西农业大学/江西省森林培育重点实验室,南昌330045 [2]中国林业科学研究院森林生态环境与保护研究所/国家林业局森林生态环境重点实验室,北京100091 [3]江西省安福县陈山林场,吉安343200

出　　处：《应用与环境生物学报》2016年第3期510-517,共8页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(31560204);国家林业局林业公益性行业科研专项(201104058);江西省高等学校科技落地计划项目(KJLD13024)资助~~

摘　　要：以不同年龄(5年、10年、20年和40年)陈山红心杉林为研究对象,采用磷脂脂肪酸(PLFA)法分析其土壤微生物群落结构与多样性.结果发现,陈山红心杉林土壤总PLFAs及各类群微生物PLFAs含量随年龄增加先下降后上升,真菌/细菌(F/B)变化不明显,G^+/G^-和SAT/MONO呈下降趋势,Cy/Pre及微生物群落多样性指标总体呈先升后降的趋势.林龄改变了土壤微生物群落结构,第一、二主成分贡献率分别为72.2%和25.0%,主要由不同林龄土壤理化性质引起.速效养分影响大于全量养分.Cy/Pre只在10年林龄大于0.5,反映环境胁迫可能导致了微生物PLFAs含量从10年林龄到20年下降,但这并未改变土壤生态系统的稳定程度.综上,不同林龄陈山红心杉林土壤微生物群落结构可反映土壤环境状况及养分可利用性,对陈山红心杉的丰产、高效、优质经营具有重要意义.(图4表3参39)Soil microbial community plays an important role in the terrestrial ecosystem by carrying out almost all soil biochemical processes. This research aimed to study the forest soil microbial community through the Chenshan Red-heart Chinese fir of different ages （5a, 10a, 20a and 40a） by using phosphilipid fatty acid （PLFA） method which has been widely used to detect soil microbial community with the advantage of simple operation, low experiment conditions and good testing stability. The results showed that with the increase of age, the content of total PLFAs and PLFAs of each kind of microbe first decreased and then increased; the PLFAs content of fungi had no obvious change; the ratio of G＋/G- and SAT/MONO decreased; that of Cy/Pre and soil microbial community diversity indexes first increased and then decreased. The changes of soil microbial community with the stand age were mainly caused by the differences in soil physical and chemical properties among different ages, with contributory ratio of PC1 and PC2 as 72.2% and 25.0% respectively. Available nutrients had larger effect than total nutrients, which reflected the impact of soil nutrient availability on microbial community. Cy/Pre was less than 0.5 except in 10a soil, indicating the existence of environment stresses （e.g. nutrient and oxygen） which may cause the decrease in microbial PLFAs from 10a to 20a. Such environment stresses did not change the stability of soil ecosystem. Therefore we should pay more attention to the soil environment condition in forests older than 10a, to prevent the adverse impacts on ecosystem.

关 键 词：土壤微生物 群落结构 林龄 磷脂脂肪酸 陈山红心杉 

分 类 号：S714.3[农业科学—林学]